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DIRECT TRANSDUCTION OF PRIMER EXTENSION INTO ELECTRICAL SIGNAL USING GENETIC FIELD EFFECT TRANSISTOR

文献类型: 会议论文

第一作者: Yuji Miyahara

作者: Yuji Miyahara 1 ; Toshiya Sakata 1 ;

作者机构: 1.Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan

关键词: DNA chip;genetic field effect transistor;potentiometric detection;extension reaction;single nucleotide polymorphism

会议名称: Micro total analysis systems 2004 :

主办单位:

页码: 303-305

摘要: We describe a novel method for single nucleotide polymorphism (SNP) analysis based on the genetic field effect transistor (FET), Using the genetic FET, extension reaction of allele specific oligonucleotide on the gate insulator could be directly transduced into electrical signal, which was caused by the increase of negative charges of oligonucleotides. Moreover, the SNP genotyping could be achieved using the genetic FETs in combination with allele specific extension reaction.

分类号: O657.3

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