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Self-assembled molecular gate field effect transistor for label free sialic acid detection at cell membrane

文献类型： 会议论文

第一作者： Akira Matsumoto

作者： Akira Matsumoto 1 ; Naoko Sato 2 ; Horacio Cabral 2 ; Kazunori Kataoka 2 ; Yuji Miyahara 1 ;

作者机构： 1.Biomaterials Center & International Center for Materials Nanoarchitectonics, National Institute for Materials Science

2.Department of Materials Science, The University of Tokyo

关键词： Self-assembled monolayer;Extended gate field effect transistor;Sialic acid;Phenylboronic acid

会议名称： Eurosensors Conference

主办单位：

页码： 926-929

摘要： This work describes a label free, potentiomctric method to detect cell surface sialic acid (SA) using phenylboronic acid (PBA) compound integrated into the form of self-assembled monolayer (SAM) on a field effect transistor (FET) extended gold gate electrode. Due to predominant binding between undisassociated PBA and SA at pH 7.4, we found that carboxyl anions of SA were exclusively detectable among other glycan chain constituent monosaccharides, as the change in threshold voltage (VT) of the PBA-modified FET. The technique was applied to analyses of altered SA expressions on rabbit erythrocyte as a models for diabetes. The comparative analyses revealed that the disease could be feasibly diagnosed simply by placing the cell suspensions onto the device without any labelling and enzymatic procedures.

分类号： TP212-53

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[1]NeuA O-acetylesterase activity is specific for CMP-activated O-acetyl sialic acid in Streptococcus suis serotype 2. Song, Lili,Zhou, Hui,Jin, Cheng,Cai, Xuehui,Li, Chunyang,Liang, Jingnan. 2011

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