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Label Free Potentiometric Sialic Acid Detection Applicable to Living Cell Diagnosis

文献类型： 会议论文

第一作者： Akira Matumoto

作者： Akira Matumoto 1 ; Naoko Sato 1 ; Horacio Cabral 1 ; Kazunori Kataoka 1 ; Yuji Miyahara 1 ;

作者机构： 1.Center for NanoBio Integration (CNBI), The University of Tokyo

2.Biomaterials Center and International Center for Materials Nanoarchitectonics, National Institute for Materials Science (NIMS)

会议名称： IEEE Sensors Conference

主办单位：

页码： 1807-1810

摘要： This work describes a label free, potentiometric method to detect cell surface sialic acid (SA) using phenylboronic acid (PBA) compound integrated into the form of self-assembled monolayer (SAM) on a field effect transistor (FET) extended gold gate electrode. Due to predominant binding between undisassociated PBA and SA at pH 7.4, we found that carboxyl anions of SA were exclusively detectable among other glycan chain constituent monosaccharides, as the change in threshold voltage (V_T) of the PBA-modified FET. The technique was applied to analyses of altered SA expressions on rabbit erythrocyte as a model for diabetes. The comparative analyses revealed that the disease could be feasibly diagnosed simply by placing the cell suspensions onto the device without any labelling and enzymatic procedures.

分类号： TP212-53

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