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Label-free and Electrochemical Detection of Nucleic Acids Based on Isothermal Amplification in Combination with Solid-state pH Sensor

文献类型： 会议论文

第一作者： Miyuki Tabata

作者： Miyuki Tabata 1 ; Yurika Katayama 1 ; Fahmida Mannan 2 ; Ayaka Seichi 3 ; Koji Suzuki 3 ; Tatsuro Goda 1 ; Akira Matsumoto 1 ; Yuji Miyahara 1 ;

作者机构： 1.Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University

2.Faculty of Medicine, Imperial College London

3.Department of Applied Chemsitry, Graduate School of Science and Engineering, Keio University

关键词： Biosensor;Iridium/iridium oxide;Isothermal nucleic acid amplification

会议名称： Eurosensors Conference

主办单位：

页码： 419-422

摘要： Label-free quantification methods for nucleic acids are attracting attention, since they provide potential tools in clinical applications such as disease diagnosis or prognosis observation. In this research, we developed a simpler and cost-effective electrical monitoring device of nucleic acid amplification, combining an isothermal amplification method and an Iridium/Iridium oxide (Ir/IrOx) electrode without labeling. The fabricated Ir/IrOx electrode showed ideal Nernstian response under the various pH buffer solutions, and its potential response was less affected even in the presence of charged proteins. Moreover, we successfully real-time monitored primer generation-rolling circle amplification (PG-RCA) at the 0, 10, 100, 1000 pM of target DNA by detection of released proton during amplification reaction at the constant reaction temperature. This label-free and portable device might be received considerable attention as a useful platform for a point-of-care testing in clinical use.

分类号： TP212-53

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