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A Novel Collagen Sponge for Cartilage Tissue Engineering

文献类型： 会议论文

第一作者： Guoping Chen

作者： Guoping Chen 1 ; Daisuke Akahane 2 ; Naoki Kawazoe 3 ; Yoshio Shirasaki; Junzo Tanaka; Katsuyuki Yamamoto; Tateishi Tateishi;

作者机构： 1.Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Japan

2.Division of Bioengineering and Bioinformatics, Graduate School of Information Science and Technology, Hokkaido University, Japan

3.Institute for Human and Science

关键词： scaffold;collagen sponge;tissue engineering;differentiation;MSC;chondrocyte

会议名称： Bioceramics vol.19 pt.2

主办单位：

页码： 1099,1104-

摘要： A novel collagen sponge that can protect cell leakage during cell seeding was developed by wrapping all the surfaces except the upside of a collagen sponge with membrane that has pores smaller than cell. The collagen sponge was used for three-dimensional culture of human bone marrow-derived mesenchymal stem cells (MSCs). The cells adhered to the collagen, and proliferated to fill the spaces in the sponge. The cell seeding efficiency was higher than 95%. The MSCs cultured in the collagen sponge in the chondrogenic induction medium supplemented with TGF-β3 and BMP6 expressed genes encoding type Ⅱ collagen, SOX9 and aggrecan. HE staining indicated the round morphology of differentiated cells and the extracelluler matrices were positively stained by safranin O and toluidine blue. Type Ⅱ collagen and cartilage proteoglycan were detected by immunostaining with anti-type Ⅱ collagen and anti-cartilage proteoglycan. These results suggest the chondrogenic differentiation of MSCs. The collagen sponge facilitated cell seeding and chondrogenic differentiation of MSCs, and will be useful for cartilage tissue engineering.

分类号： [ "R318.08" , "R318.08"]

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