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Time-controlled dual-stage strategy for bioproduction of high-purity (R-R) 2,3-butanediol and acetoin in Bacillus licheniformis

文献类型： 外文期刊

作者： He, Hehe 1 ; Xu, Yinbiao 3 ; Li, Siyu 4 ; Deng, Xiangyuan 1 ; Shi, Guiyang 4 ; Li, Youran 4 ;

作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biotechnol, Jiangsu Key Lab Sericultural & Anim Biotechnol, Zhenjiang 212100, Peoples R China

2.Chinese Acad Agr Sci, Sericultural Sci Res Ctr, Key Lab Silkworm & Mulberry Genet Improvement, Minist Agr & Rural Affairs, Zhenjiang 212100, Peoples R China

3.Henan Univ, Inst Microbial Engn, Sch Life Sci, Kaifeng 475004, Peoples R China

4.Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, 1800 Lihu Ave, Wuxi 214000, Jiangsu, Peoples R China

5.Jiangnan Univ, Natl Engn Res Ctr Cereal Fermentat & Food Biomfg, 1800 Lihu Ave, Wuxi 214000, Jiangsu, Peoples R China

6.Jiangnan Univ, Jiangsu Provis Res Ctr Bioact Prod Proc Technol, 1800 Lihu Ave, Wuxi 214122, Jiangsu, Peoples R China

关键词： 2,3-Butanediol; Acetoin; Bioproduction; Bacillus licheniformis; Bioseparation

期刊名称：FOOD BIOSCIENCE （ 影响因子：5.9； 五年影响因子：6.1 ）

ISSN： 2212-4292

年卷期： 2025 年 63 卷

页码：

收录情况： SCI

摘要： (R-R) 2,3-butanediol (BDO) is a chemical compound of significant medical value, and there is a desire to biologically produce high-purity (R-R) 2,3-BDO instead of a mixture of various configurations. Acetoin, a key downstream metabolite, gains attention for food industry applications. Simultaneous production through a single fermentation holds great potential, but the absence of a convenient and efficient separation strategy remains a source of disappointment. Here, we meticulously chose non-pathogenic Bacillus licheniformis as cell factories with specific genetic regulation on this metabolic pathway. Firstly, genome engineering was performed to attain 100% purity in the production of (R-R) 2,3-BDO. Subsequently, by harnessing the inherent regulation of the glyoxylate cycle within the strain, a pioneering two-stage production strategy was developed. This strategy enables selective production of high-purity (R-R) 2,3-BDO or acetoin based on production preferences, simply by terminating fermentation at the appropriate time. When using 80 g/L of glucose as the substrate, it is feasible to obtain 22.0 g/L of (R-R) 2,3-BDO with 100% purity or 26.0 g/L of acetoin with 90.0% purity. In summary, this work presents an inspiring strategy that addresses dual production for a better product separation, which can serve as a source of inspiration for other cell factories in the field of biomanufacturing.

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