您好,欢迎访问中国农业科学院 机构知识库!

Cloning, characterization, and expression analysis of a novel BmGDAP1 gene from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection

文献类型: 外文期刊

作者: Gao, Kun 1 ; Deng, Xiangyuan 4 ; Qian, Heying 1 ; Wu, Ping 1 ; Qin, Guangxing 1 ; Guo, Xijie 1 ;

作者机构: 1.Jiangsu Univ Sci & Technol, Sericultural Res Inst, Zhenjiang 212018, Jiangsu, Peoples R China

2.Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang 212018, Jiangsu, Peoples R China

3.Jiangsu Univ Sci & Technol, Coll Biotechnol & Chem Engn, Zhenjiang 212003, Jiangsu, Peoples R China

4.Jia

关键词: Bombyx mori;Cytoplasmic polyhedrosis virus;Expression analysis;Ganglioside-induced differentiation-associated protein 1;Midgut;Rapid amplification of cDNA ends

期刊名称:GENE ( 影响因子:3.688; 五年影响因子:3.329 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A novel ganglioside-induced differentiation-associated protein 1 gene (BmGDAP1) was first cloned and sequenced from silkworm, Bombyx mori using rapid amplification of cDNA ends (RACE). The full-length cDNA of BmGDAP1 was 1514. bp, consisting of a 91. bp 5' untranslated region (UTR), a 424. bp 3'-UTR and a 999. bp open reading frame (ORF). The ORF encoded a polypeptide of 332 amino acids, which possessed a thioredoxin (TRX)-like domain, a glutathione S-transferase-C (GST-C) family domain and a transmembrane segment. Furthermore, quantitative real-time PCR analysis revealed that BmGDAP1 transcripts were mainly presented in the tissues of hemocytes and midgut of silkworm, and its expression level was down-regulated in the hemocytes, while up-regulated in the midgut. Therefore, it could be concluded that BmGDAP1 plays an important role in the recognition and immune response of silkworm to BmCPV infection.

  • 相关文献

[1]Cloning and characterization of the gene encoding an ubiquitin-activating enzyme E1 domain-containing protein of silkworm, Bombyx mori. Wu, Ping,Li, Mu-Wang,Guo, Xi-Jie,Jiang, Yun-Feng,Wang, Zi-Sheng,Guo, Xi-Jie. 2010

[2]Novel protein of IBP from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection. Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Shen, Zhong-yuan,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Shen, Zhong-yuan,Guo, Xi-jie,Gao, Kun,Deng, Xiang-yuan. 2012

[3]Molecular cloning and characterization of biphenyl hydrolase-like (BPHL) protein gene from silkworm, Bombyx mori. Gao, Kun,Deng, Xiang Yuan,Gao, Kun,Shang, Meng Ke,Qian, He Ying,Guo, Xi Jie,Gao, Kun,Shang, Meng Ke,Qian, He Ying,Guo, Xi Jie. 2016

[4]Differentially expressed genes in the midgut of Silkworm infected with cytoplasmic polyhedrosis virus. Wu, Ping,Li, Mu-Wang,Wang, Xiu-Ye,Liu, Ting,Qin, Guang-Xing,Guo, Xi-Jie,Wang, Xiu,Zhao, Pan,Guo, Xi-Jie. 2009

[5]Digital Gene Expression analysis in the midgut of 4008 silkworm strain infected with cytoplasmic polyhedrosis virus. Gao, Kun,Deng, Xiang-yuan,Gao, Kun,Qjan, He-ying,Qin, Guangxing,Guo, Xi-jie,Gao, Kun,Qjan, He-ying,Qin, Guangxing,Guo, Xi-jie. 2014

[6]iTRAQ-based quantitative proteomic analysis of midgut in silkworm infected with Bombyx mori cytoplasmic polyhedrosis virus. Gao, Kun,Deng, Xiang-Yuan,Shang, Meng-Ke,Qin, Guang-Xing,Hou, Cheng-Xiang,Guo, Xi-Jie.

[7]Molecular cloning and characterization of High Mobility Group box (HMGB) gene from Beauveria bassiana- infected silkworm, Bombyx mori. Chengxiang, H.,Han, W.,Dingding, L.,Ruilin, L.,Xijie, G.,Chengxiang, H.,Han, W.,Dingding, L.,Ruilin, L.,Xijie, G.. 2017

[8]Microarray analysis of the gene expression profile in the midgut of silkworm infected with cytoplasmic polyhedrosis virus. Wu, Ping,Wang, Xiu,Jiang, Yun-Feng,Guo, Xi-Jie,Wu, Ping,Qin, Guang-xing,Liu, Ting,Li, Mu-Wang,Guo, Xi-Jie.

[9]Cloning and expression analysis of a peptidoglycan recognition protein in silkworm related to virus infection. Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie,Gao, Kun,Deng, Xiang-yuan.

[10]Cytoplasmic polyhedrosis virus-induced differential gene expression in two silkworm strains of different susceptibility. Gao, Kun,Deng, Xiang-yuan,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie.

[11]CDNA cloning and characterization of LASP1 from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection. Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Guo, Xi-jie,Gao, Kun,Deng, Xiang-yuan.

[12]The physiological differentiation along the midgut of Bombyx mori - inspirations from proteomics and gene expression patterns of the secreted proteins in the ectoperitrophic space. Qu, M.,Yang, J.,Yang, Q.,Liu, L.,Qu, M.,Yang, J.,Yang, Q.,Yang, Q.. 2018

[13]Early responses of silkworm midgut to microsporidium infection - A Digital Gene Expression analysis. Yue, Ya-Jie,Tang, Xu-Dong,Xu, Li,Yan, Wei,Li, Qian-Long,Xiao, Sheng-Yan,Fu, Xu-Liang,Wang, Wei,Li, Nan,Shen, Zhong-Yuan,Yue, Ya-Jie,Tang, Xu-Dong,Xu, Li,Yan, Wei,Li, Qian-Long,Xiao, Sheng-Yan,Fu, Xu-Liang,Wang, Wei,Li, Nan,Shen, Zhong-Yuan,Yue, Ya-Jie,Tang, Xu-Dong,Xiao, Sheng-Yan,Li, Nan,Shen, Zhong-Yuan.

[14]Down-regulation of aminopeptidase N and ABC transporter subfamily G transcripts in Cry1Ab and Cry1Ac resistant Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae). Wang, Yueqin,Wang, Yidong,Bai, Shuxiong,Wang, Zhenying,He, Kanglai,Coates, Brad S.. 2017

[15]Activities of Digestive Enzymes in the Omnivorous Pest Apolygus lucorum (Hemiptera: Miridae). Yuan, Wei,Wu, Kongming,Zhao, Xincheng.

[16]Reduction of Bacillus thuringiensis Cry1Ac toxicity against Helicoverpa armigera by a soluble toxin-binding cadherin fragment. Liu, Chenxi,Wu, Kongming,Gao, Yulin,Ning, Changming,Wu, Yidong,Ning, Changming,Oppert, Brenda.

[17]Tissue-specific transcriptome profiling of Plutella xylostella third instar larval midgut. Xie, Wen,Lei, Yanyuan,Fu, Wei,Yang, Zhongxia,Zhu, Xun,Guo, Zhaojiang,Wu, Qingjun,Wang, Shaoli,Xu, Baoyun,Zhang, Youjun,Zhou, Xuguo.

[18]Genomic organization, differential expression, and functional analysis of the SPL gene family in Gossypium hirsutum. Zhang, Xiaohong,Dou, Lingling,Pang, Chaoyou,Song, Meizhen,Wei, Hengling,Fan, Shuli,Wang, Chengshe,Yu, Shuxun. 2015

[19]Genome-wide identification and functional analysis of the TIFY gene family in response to drought in cotton. Ge Zhao,Yun Song,Caixiang Wang,Hamama Islam Butt,Qianhua Wang,Chaojun Zhang,Zuoren Yang,Zhao Liu,Eryong Chen,Xueyan Zhang,Fuguang Li.

[20]Genome-wide identification and characterization of SnRK2 gene family in cotton (Gossypium hirsutum L.). Zhao Liu,Xiaoyang Ge,Zuoren Yang,Li, Fuguang,Zhang, Xueyan,Chaojun Zhang,Ge Zhao,Eryong Chen,Ji Liu,Xueyan Zhang,Fuguang Li. 2017

作者其他论文 更多>>