Cloning and expression analysis of a detoxification enzyme BmmGSTo3 gene in wild silkworm, Bombyx mandarina (Lepidoptera: Bombycidae)
文献类型： 外文期刊
作者： Li, Ruonan 1 ; Chen, Enxi 1 ; Zhang, Yuming 1 ; Wang, Mengjiao 1 ; Zhu, Lin 1 ; Qian, Yixuan 1 ; Zhao, Guodong 1 ; Qian, Heying 1 ;
作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biotechnol, Jiangsu Key Lab Sericultural Biol & Biotechnol, 666 Changhui Rd, Zhenjiang 212100, Peoples R China
2.Minist Agr & Rural Affairs, Chinese Acad Agr Sci, Sericultural Res Inst, Key Lab Silkworm & Mulberry Genet Improvement, Zhenjiang 212100, Peoples R China
关键词： BmNPV; insecticide; oxidative stress; virus; glutathione S-transferase
期刊名称：EUROPEAN JOURNAL OF ENTOMOLOGY （ 影响因子：1.2； 五年影响因子：1.2 ）
ISSN：
年卷期： 2025 年 122 卷
页码：
收录情况： SCI
摘要： Bombyx mandarina is one of prevalent pests in mulberry gardens during summer and autumn, posing signifi cant challenges to sericulture across China. Glutathione S-transferase (GST), a vital detoxifi cation enzyme in insects, plays a crucial role in protecting organisms from DNA damage and oxidative stress. In this study, the GSTo3 gene, coding glutathione S-transferase of B. mandarina, was cloned and its physicochemical properties and structures were predicted using bioinformatics tools. The relative expression levels in various tissues and induced transcriptional levels were detected by using qRT-PCR. Results revealed a 99.17% sequence similarity of GSTo3 gene between B. mandarina and Bombyx mori. It is mainly distributed in the head, fat body and epidermis tissues of B. mandarina larvae. After BmNPV infection, elevated GSTo3 expression levels were observed in the midgut. The transcriptional levels of GSTo3 gene were signifi cantly up-regulated after exposure to phoxim, deltamethrin and chlorfenapyr exposure respectively in the fat body and midgut of B. mandarina. These fi ndings underscore the signifi cance of GSTo3 gene in exogenous materials metabolism and provide a new perspective on the resistance mechanism in B. mandarina.
- 相关文献
作者其他论文 更多>>
-
Bombyx mori PAT4 gene inhibits BmNPV infection and replication through autophagy
作者：Jia, Kaifang;Wang, Jinyang;Jiang, Dan;Ding, Xiangrui;Zhao, Qiaoling;Shen, Dongxu;Qiu, Zhiyong;Zhang, Xuelian;Qian, Heying;Xia, Dingguo;Jia, Kaifang;Wang, Jinyang;Jiang, Dan;Ding, Xiangrui;Zhao, Qiaoling;Shen, Dongxu;Qiu, Zhiyong;Zhang, Xuelian;Qian, Heying;Xia, Dingguo;Lu, Cheng
关键词：Antiviral; BmNPV; Bombyx mori; PAT4; Cellular autophagy; BmTORC1
-
Ethanol inhibited growth hormone receptor-mediated endocytosis in primary mouse hepatocytes
作者：Ge, Zihan;Liu, Xingjie;Zhang, Wei;Xian, Li;Li, Ruonan;Ge, Zihan;Yang, Yu;Lan, Hainan;Zhang, Yawen
关键词：growth hormone; growth hormone receptor; ethanol; nuclear translocation; endocytosis
-
Effects of Novaluron Exposure on the Oviposition and Expression of Ovarian Development Related Genes in Silkworm, Bombyx mori (Lepidoptera: Bombycidae)
作者：Wang, Meng-Jiao;Chen, En-Xi;Ji, Yi-Lin;Qian, Yi-Xuan;Zhang, Yu-Ming;Zhu, Lin;Zhao, Guo-Dong;Qian, He-Ying;Zhao, Guo-Dong;Qian, He-Ying
关键词：silkworm; novaluron; oviposition; ovary development; hormone regulation
-
BmWARS inhibits BmNPV infection via the PI3K-Akt pathway
作者：Wang, Jinyang;Ding, Xiangrui;Jia, Kaifang;Chen, Haiyu;Zhao, Qiaoling;Shen, Dongxu;Qiu, Zhiyong;Zhang, Xuelian;Qian, Heying;Xia, Dingguo;Wang, Jinyang;Ding, Xiangrui;Jia, Kaifang;Chen, Haiyu;Zhao, Qiaoling;Shen, Dongxu;Qiu, Zhiyong;Zhang, Xuelian;Qian, Heying;Xia, Dingguo;An, Guorong
关键词：Antiviral;
BmWARS ; BmNPV; PI3k-Akt; Cell proliferation and apoptosis -
Influence of different temperatures and diets on the life cycle of invasive species Conogethes punctiferalis
作者：Ramzan, Muhammad;Shi, Longfei;Pang, Tianyuan;Chen, Xiangzhi;Li, Ruonan;Zhang, Yongjun;Pang, Tianyuan;Almaary, Khalid S.
关键词：climatic conditions; Conogethes punctiferalis; host plants; invasive species; life history
-
OsMYB1 antagonizes OsSPL14 to mediate rice resistance to brown planthopper and Xanthomonas oryzae pv. oryzae
作者：Sun, Bo;Sun, Bo;Sun, Bo;Zhong, Yuan;Tao, Zhihuan;Zhu, Lin;Miao, Xuexia;Li, Haichao;Zhong, Yuan;Tao, Zhihuan;Zhu, Lin;Shi, Zhenying
关键词：MYB; SPL; Brown planthopper;
Xanthomonas oryzae pv. oryzae -
EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research
作者：Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang
关键词：metagenome; microbiome; microbiota; pipeline; visualization
