您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Multi-omics profiling reveals infectious bursal disease virus-induced alterations in gene expression and metabolism in chicken bursa of fabricius

文献类型： 外文期刊

作者： Song, Cuiping 1 ; Li, Siyu 1 ; Yuan, Yanmei 1 ; Gao, Wei 3 ; Qiu, Xusheng 1 ; Tan, Lei 1 ; Sun, Yingjie 1 ; Tang, Ning 1 ; Qu, Yang 1 ; Liao, Ying 1 ; Ding, Chan 1 ;

作者机构： 1.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Ziyue Rd 518, Shanghai 200241, Peoples R China

2.Guangxi Univ, Coll Anim Sci & Vet Med, Nanning, Guangxi, Peoples R China

3.Yangzhou Univ, Coll Vet Med, Wenhui Rd 48, Yangzhou 225009, Peoples R China

4.Shanghai Jiao Tong Univ, Coll Agr & Biol, Dongchuan Rd 800, Shanghai 200240, Peoples R China

关键词： Infectious bursal disease virus; Transcriptomics; Metabolomics; Arachidonic acid; NLRP3

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：2.7； 五年影响因子：2.9 ）

ISSN： 0378-1135

年卷期： 2025 年 308 卷

页码：

收录情况： SCI

摘要： Infectious Bursal Disease (IBD) is an acute, highly contagious disease caused by IBDV, characterized by inflammation, atrophy of the Bursa of Fabricius, and immunosuppression. This study infected 21-day-old SPF chickens with three IBDV strains (classical YZ, very virulent AH, and variant SD). On day 7 post-infection, bursa samples were collected for transcriptomic and metabolomic analyses. Metabolite profiles were analyzed using multivariate statistics, and KEGG enrichment analysis was used to identify dysregulated pathways, elucidating the transcriptional and metabolic responses in IBDV-infected bursa tissue. Transcriptomic analysis identified 1733 DEGs in the YZ group, 5731 in the AH group, and 84 in the SD group. Gene Ontology clustering of common SDE genes between virus-infected and control groups focused on cellular components, molecular functions, and biological processes;KEGG enrichment showed they were mainly involved in lipid-related metabolic pathways for the three IBDV subtypes. Metabolomic analysis detected 460 significantly changed metabolites per subtype after IBDV infection. Lipid metabolism disorders were associated with IBDV, involving L-carnitine and other substances;KEGG analysis indicated the main pathways were lipid-related, like arachidonic acid (AA) metabolism. Moreover, this study verified mRNA levels of cytokines, NLRP3 protein level, and AA content. IBDV infection induces differential gene expression related to host immune response and metabolic regulation at the transcriptomic level, with metabolomic changes mainly involving lipid metabolism. Integrating transcriptomics and metabolomics provides a comprehensive understanding of the host's response to IBDV infection.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台