您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae

文献类型： 外文期刊

作者： Cheng, Li 1 ; Zhao, Shijun 1 ; Li, Tianyi 1 ; Hou, Sha 1 ; Luo, Zhouqing 1 ; Xu, Jinsheng 4 ; Yu, Wenfei 1 ; Jiang, Shuangying 1 ; Monti, Marco 5 ; Schindler, Daniel 5 ; Zhang, Weimin 6 ; Hou, Chunhui 8 ; Ma, Yingxin 1 ; Cai, Yizhi 1 ; Boeke, Jef D. 6 ; Dai, Junbiao 1 ;

作者机构： 1.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Key Lab Quantitat Synthet Biol,Shenzhen Key Lab Sy, Shenzhen 518055, Peoples R China

2.Univ Chinese Acad Sci, Beijing, Peoples R China

3.Xiamen Univ, Innovat Ctr Cell Signaling Network, Sch Life Sci, State Key Lab Cellular Stress Biol, Xiamen 361102, Fujian, Peoples R China

4.Huazhong Agr Univ, Dept Bioinformat, Wuhan 430070, Hubei, Peoples R China

5.Univ Manchester, Manchester Inst Biotechnol, Manchester M1 7DN, England

6.NYU Langone Hlth, Inst Syst Genet, New York, NY USA

7.NYU Langone Hlth, Dept Biochem & Mol Pharmacol, New York, NY USA

8.Chinese Acad Sci, Kunming Inst Zool, China State Key Lab Genet Resources & Evolut, Kunming 650223, Peoples R China

9.NYU Tandon Sch Engn, Dept Biomed Engn, Brooklyn, NY 11201 USA

10.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch, Minist Agr & Rural Affairs,Guangdong Lab Lingnan M, Shenzhen, Peoples R China

11.Shenzhen Univ, Coll Life Sci & Oceanog, 1066 Xueyuan Rd, Shenzhen 518055, Guangdong, Peoples R China

12.Shenzhen Lianghe Biotechnol Co Ltd, Shenzhen, Peoples R China

期刊名称：NATURE COMMUNICATIONS （ 影响因子：16.6； 五年影响因子：17.0 ）

ISSN：

年卷期： 2024 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution (SCRaMbLE) is a promising tool to study genomic rearrangements. However, the potential of SCRaMbLE to study genomic rearrangements is currently hindered, because a strain containing all 16 synthetic chromosomes is not yet available. Here, we construct SparLox83R, a yeast strain containing 83 loxPsym sites distributed across all 16 chromosomes. SCRaMbLE of SparLox83R produces versatile genome-wide genomic rearrangements, including inter-chromosomal events. Moreover, when combined with synthetic chromosomes, SCRaMbLE of hetero-diploids with SparLox83R leads to increased diversity of genomic rearrangements and relatively faster evolution of traits compared to hetero-diploids only with wild-type chromosomes. Analysis of the SCRaMbLEd strain with increased tolerance to nocodazole demonstrates that genomic rearrangements can perturb the transcriptome and 3D genome structure and consequently impact phenotypes. In summary, a genome with sparsely distributed loxPsym sites can serve as a powerful tool for studying the consequence of genomic rearrangements and accelerating strain engineering in Saccharomyces cerevisiae.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台