文献类型： 外文期刊
作者： Su, Cong 1 ; Cui, Haotian 2 ; Wang, Weiwei 2 ; Liu, Yong 1 ; Cheng, Zhenyu 2 ; Wang, Chen 1 ; Yang, Mengqiao 2 ; Qu, Liwen 1 ; Li, Ye 2 ; Cai, Yuejin 4 ; He, Siyang 2 ; Zheng, Jiaxin 4 ; Zhao, Pingping 4 ; Xu, Ping 2 ; Dai, Junbiao 1 ; Tang, Hongzhi 2 ;
作者机构： 1.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Shenzhen Key Lab Synthet Genom,Guangdong Prov Key, Shenzhen 518055, Peoples R China
2.Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai, Peoples R China
3.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai, Peoples R China
4.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Shenzhen Key Lab Agr Synthet Biol,Agr Genom Inst S, Shenzhen Branch,Guangdong Lab Lingnan Modern Agr,G, Shenzhen 518124, Peoples R China
期刊名称：NATURE （ 影响因子：48.5； 五年影响因子：55.0 ）
ISSN： 0028-0836
年卷期： 2025 年 642 卷 8069 期
页码：
收录情况： SCI
摘要： Industrial wastewater, petroleum pollution and plastic contamination are significant threats to global marine biosecurity because of their toxic, mutagenic and persistent nature1. The use of microorganisms in bioremediation has been constrained by the complexity of organic pollutants and limited tolerance to saline stress2. In this study, we used synthetic biology to engineer Vibrio natriegens into a strain capable of bioremediating complex organic pollutants in saline wastewater and soils. The competence master regulator gene tfoX was inserted into chromosome 1 of the V. natriegens strain Vmax and overexpressed to enhance DNA uptake and integration. Degradation gene clusters were chemically synthesized and assembled in yeast. We developed a genome engineering method (iterative natural transformation based on Vmax with amplified tfoX effect) to transfer five gene clusters (43 kb total) into Vmax. The engineered strain has the ability to bioremediate five organic pollutants (biphenyl, phenol, naphthalene, dibenzofuran and toluene) covering a broad substrate range, from monocyclic to multicyclic compounds, in industrial wastewater samples from a chlor-alkali plant and a petroleum refinery.
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