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Metagenomic Investigation of Pathogenic RNA Viruses Causing Diarrhea in Sika Deer Fawns

文献类型： 外文期刊

作者： Wang, Weiyang 1 ; Wang, Qilin 1 ; Cao, Runlai 1 ; Li, Yacong 1 ; Liu, Ziyu 1 ; Xue, Zhuqing 1 ; Wang, Xiaoxu 1 ; Liu, Zhijie 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Special Anim & Plant Sci, Minist Agr & Rural Affairs, Key Lab Special Anim Epidem Dis,Jilin Prov Int Coo, Changchun 130112, Peoples R China

关键词： sika deer; viral metagenomics; whole-genome sequencing; rotavirus

期刊名称：VIRUSES-BASEL （ 影响因子：3.5； 五年影响因子：3.7 ）

ISSN：

年卷期： 2025 年 17 卷 6 期

页码：

收录情况： SCI

摘要： Diarrhea is a common disease in sika deer. The causes of diarrhea in sika deer are complex and involve a variety of pathogens. Additionally, new virulent pathogens are continuously emerging, which poses a serious threat to deer's health and particularly affects fawns' survival rate. In the present study, feces samples were collected from fawns with diarrhea in Jilin Province, in the northeast of China. The viral communities were investigated using the metagenomic method. Viral metagenome data revealed that the viruses in the fecal samples were mainly from 21 families in 14 orders. The major viruses in high abundance were astrovirus, rotavirus, coronavirus, and bovine viral diarrhea virus. In addition, a large number of phages, which mainly belonged to the family Siphoviridae, were identified. Then, the known causative virus species were investigated via RT-qPCR. The results showed that the infection rates of bovine coronavirus, bovine rotavirus, and bovine viral diarrhea virus were 59.44%, 58.89%, and 21.67%, respectively, and mixed infections were commonly seen in the samples. A bovine rotavirus strain was successfully isolated from the positive samples. Whole-genome sequencing revealed that the genotype of the strain was G6-P[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3, indicating the recombination of rotavirus. This study revealed the profiles and characteristics of viruses that cause sika deer diarrhea, which will be helpful for understanding diarrhea diseases in sika deer.

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