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Maturation of Eupyrene Sperm upon Ejaculation Is Influenced by a Male Accessory Gland-Derived Serine Protease in Grapholita molesta

文献类型： 外文期刊

作者： Cheng, Jie 1 ; Guo, Tai 1 ; Zhou, Zhongyan 1 ; Wei, Wei 1 ; Liang, Yu 1 ; Xiang, Huiming 1 ; Ma, Ruiyan 1 ; Shen, Zhongjian 3 ; Zhao, Zhi-Guo 1 ;

作者机构： 1.Shanxi Agr Univ, Coll Plant Protect, Taigu 030801, Peoples R China

2.Shanxi Key Lab Bioagent Utilizat & Ecopesticide In, Taigu 030801, Peoples R China

3.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Key Lab Nat Enemy Insects,Minist Agr & Rural Affai, Beijing 100193, Peoples R China

关键词： oriental fruit moth; serine protease; male fertility; eupyrene sperm; spermatophore; metabolic signatures

期刊名称：INSECTS （ 影响因子：2.9； 五年影响因子：3.3 ）

ISSN：

年卷期： 2025 年 16 卷 8 期

页码：

收录情况： SCI

摘要： Grapholita molesta is a globally significant fruit pest. Females achieve maximal reproductive output through efficient sperm utilization following a single copulation. Post-mating maturation of eupyrene sperm is a critical step in reproductive success. Here, we report that a male accessory gland-derived serine protease (named GmAGSP1) is essential for this process. GmAGSP1 was only distantly related to other identified sperm-activating SPs, and its transcript was highly expressed in the AG at 48 h after emergence. RNAi-mediated knockdown of GmAGSP1 in males did not affect courtship rate, copulation duration, or mating frequency, whereas male fertility decreased significantly. Mating with GmAGSP1-knockdown males markedly impaired eupyrene sperm maturation in the spermatophores, with phenotypes including failure of eupyrene sperm bundles to dissociate normally and marked reduction in viability of the dissociated eupyrene sperm. Finally, untargeted metabolomic analysis preliminarily demonstrated marked alterations in multiple metabolic pathways within the spermatophore following mating with GmAGSP1-knockdown males. This study advances our understanding of the regulatory mechanism of "sperm activation in the spermatophore's metabolic microenvironment mediated by male AG-derived SP" while providing critical insights for the development of novel genetic control strategies targeting G. molesta.

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