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Proteomics study on the protective mechanism of soybean isoflavone against inflammation injury of bovine mammary epithelial cells induced byStreptococcus agalactiae

文献类型： 外文期刊

作者： Niu, Hui 1 ; Zhang, Hua 1 ; Wu, Fuxin 1 ; Xiong, Benhai 2 ; Tong, Jinjin 1 ; Jiang, Linshu 1 ;

作者机构： 1.Beijing Agr Univ, Dept Anim Sci, Anim Sci & Technol Coll, Beijing 102206, Peoples R China

2.Chinese Acad Agr Sci, State Key Lab Anim Nutr, Inst Anim Sci, Beijing 100193, Peoples R China

关键词： Soybean isoflavone; Dairy mammary epithelial cells; Streptococcus agalactiae; Protection mechanism; Proteomics

期刊名称：CELL STRESS & CHAPERONES （ 影响因子：3.667； 五年影响因子：3.717 ）

ISSN： 1355-8145

年卷期：

页码：

收录情况： SCI

摘要： This study aimed to verify the anti-inflammatory effect of soybean isoflavones (SI) on the inflammatory response induced byStreptococcus agalactiae(S. agalactiae) of bovine mammary epithelial cells (bMECs) and to elucidate its possible mechanism. BMECs were pretreated with SI of different concentrations (20, 40, 60, 80, 100 mu g/mL) for 0.5, 3, 6, 9, 12, 15, 18, 24 h. And then,S. agalactiaewas used to infect bMECs for 6 h (MOI = 50:1) to establish the inflammation model. Cell viability, growth curves ofS. agalactiae, cytotoxicity, andS. agalactiaeinvasion rate were determined. A proteomics technique was used to further detect differential proteins and enrichment pathways. SI (40 mu g/mL) improved the viability of bMECs at 12 h (p < 0.05) and 60 and 80 mu g/mL of SI greater (p < 0.01). Moreover, 60 mu g/mL of SI protects cells from bacterial damage (p < 0.05). SI could inhibitS. agalactiaegrowth and internalization into bMECs in a time- and dose-dependent manner. In addition, proteomics results showed that 133 proteins were up-regulated and 89 proteins were down-regulated significantly. The differentially significantly expressed proteins (DSEPs) were mainly related to cell proliferation, differentiation, apoptosis, and migration. GO annotation showed that 222 DSEPs were divided into 23 biological processes (BP) terms, 14 cell components (CC) terms, and 12 molecular functions (MF) terms. DSEPs were significantly enriched in 10 pathways, of which the immune pathway was the main enrichment pathway.

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