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Vertical flow immunoassay for multiplex mycotoxins based on photonic nitrocellulose and SERS nanotags

文献类型： 外文期刊

作者： Chen, Ruipeng 1 ; Hu, Jingfang 4 ; Wang, Hui 1 ; Li, Cheng 5 ; Kang, Haiqi 6 ; Chen, Yuxuan 1 ; Yang, Liang 1 ; Tang, Xiangfang 1 ; Xiong, Benhai 1 ; Zhao, Xiangwei 2 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr & Feeding, Beijing 100193, Peoples R China

2.Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Digital Med Engn, Nanjing 210096, Peoples R China

3.Southeast Univ, Shenzhen Res Inst, Shenzhen 518000, Peoples R China

4.Beijing Informat Sci & Technol Univ, Beijing Key Lab Sensor, Beijing 100101, Peoples R China

5.Chinese Acad Agr Sci, Inst Food Sci & Technol, Beijing 100193, Peoples R China

6.Beijing Univ Agr, Coll Econ & Management, Beijing 102206, Peoples R China

关键词： Surface-enhanced Raman scattering; Vertical flow assay; Photonic nitrocellulose (PNC); Slow-photon effect; Multiplex mycotoxin detection

期刊名称：FOOD CHEMISTRY-X （ 影响因子：8.2； 五年影响因子：8.2 ）

ISSN： 2590-1575

年卷期： 2025 年 25 卷

页码：

收录情况： SCI

摘要： Here, we report a SERS based VFA using PNC as a sensing substrate for highly sensitive multiplex mycotoxins detection. The PNC was fabricated by filtration-based self-assembled monodisperse SiO2 NPs on a filter membrane as a template, and the obtained PNC had an ordered complementary inverse opal structure. In parallel, three kinds of Raman dyes encoding Au@NBAAg, Au@4-MBAAg and Au@DNTBAg SERS nanotags were synthesized for the detection of OTA, AFB1 and ZON. In the immunoassay, because of the slow-photon effect of the PNC substrate, which facilitates the light coupling with SERS nanotags, the SERS signal was highly enhanced. The LODs of 8.2, 13.7 and 47.6 fg mL- 1 were achieved for simultaneous detection of OTA, AFB1 and ZON, respectively, which were lower than the tolerable cutoff values recommended by the EC. Therefore, this proposed PNC based SERS VFA is expected to be a powerful method for multiplex mycotoxins detection.

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