您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

CRISPR/Cas9-mediated gene editing of MsCLE3a confers compact alfalfa architecture

文献类型： 外文期刊

作者： Li, Hefan 1 ; Li, Cheng 1 ; Pedro, Garcia-Caparros 2 ; Zhang, Qian 3 ; Jiang, Hua 4 ; Yang, Yongping 1 ; Duan, Yuanwen 1 ; Sun, Xudong 1 ;

作者机构： 1.Chinese Acad Sci, Inst Tibetan Plateau Res Kunming, Kunming Inst Bot, Yunnan Key Lab Crop Wild Relat Om,Germplasm Bank W, Kunming 650201, Yunnan, Peoples R China

2.Univ Almeria, Higher Engn Sch, Almeria 04120, Spain

3.Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Lanzhou 730050, Peoples R China

4.Yunnan Agr Univ, Fac Anim Sci & Technol, Kunming 650201, Yunnan, Peoples R China

5.Univ Chinese Acad Sci, Beijing, Peoples R China

6.Chinese Acad Sci, Kunming Inst Bot, Yunnan Lijiang Forest Ecosyst Natl Observat & Res, Lijiang 674100, Yunnan, Peoples R China

关键词： Cell wall; Leaf petiole angle; Medicago sativa; MsCLE3; Plant architecture

期刊名称：PLANT SCIENCE （ 影响因子：4.1； 五年影响因子：5.1 ）

ISSN： 0168-9452

年卷期： 2025 年 357 卷

页码：

收录情况： SCI

摘要： Alfalfa (Medicago sativa L.) is a globally major forage crop for livestock due to its superior nutritional value and palatability. As a premium forage species, alfalfa breeding has traditionally emphasized high yield. In this study, we designed guide RNA (gRNA) to target the coding sequence of the MsCLE3a gene, and constructed a CRISPR/ Cas9 vector to generate knockouts. Five mutant lines were successfully isolated, displaying upright petioles and a compact ternately compound leaf phenotype during the seedling stage. Cryo-scanning electron microscopy (Cryo-SEM) was employed to analyze cell morphology on the abaxial leaf surface. The results indicated that the cells on the abaxial side of the Mscle3a mutants were significantly elongated compared to the wild type (WT). Transcriptomic analysis further uncovered the down-regulation of brassinosteroid (BR) biosynthesis genes in Mscle3a mutants, which might underlie the mechanisms regulating petiole angle. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed a major reduction in the transcript levels of several key genes involved in BR signaling pathways in the Mscle3a mutants relative to WT. This transcriptional down-regulation was strongly associated with the observed alterations in leaf petiole angle (LPA) in the mutant lines. Our findings provide novel insights into the novel regulatory role of MsCLE3a in alfalfa development and pinpoint potential targets for manipulating LPA in crop breeding programs.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台