文献类型： 外文期刊

作者： Ou, Zhihua ¹ ; Li, Li ³ ; Ren, Peidi ¹ ; Zhou, Ting-Ting ³ ; He, Fan ¹ ; Chen, Jialing ¹ ; Cai, Huimin ² ; Han, Xiumin ⁷ ; Wu, Yao-Dong ³ ; Li, Jiandong ² ; Li, Xiu-Rong ³ ; Tan, Qiming ¹ ; Li, Wenhui ³ ; Chen, Qi ¹ ; Zhang, Nian-Zhang ³ ; He, Xiuju ² ; Chen, Wei-Gang ³ ; Zhao, Yanping ² ; Sun, Jiwen ³ ; Zhang, Qian ¹ ; Wu, Yan-Tao ³ ; Liang, Yingan ² ; You, Jie ³ ; Hu, Guohai ¹⁰ ; Tian, Xue-Qi ³ ; Liao, Sha ⁶ ; Fu, Bao-Quan ³ ; Chen, Ao ¹¹ ; Cai, Xue-Peng ³ ; Yang, Huanming ⁶ ; Wang, Jian ⁶ ; Jin, Xin ⁶ ; Xu, Xun ⁶ ; Jia, Wan-Zhong ³ ; Li, Junhua ² ; Yan, Hong-Bin ³ ;

作者机构： 1.BGI Res, Beijing 102601, Peoples R China

2.BGI Res, Shenzhen Key Lab Unknown Pathogen Identificat, Shenzhen 518083, Peoples R China

3.Lanzhou Univ, Lanzhou Vet Res Inst, Chinese Acad Agr Sci, State Key Lab Anim Dis Control & Prevent,Coll Vet, Lanzhou 730046, Peoples R China

4.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Key Lab Ruminant Dis Prevent & Control West, Natl Para Reference Lab Anim Echinococcosis,Minist, Lanzhou 730046, Peoples R China

5.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China

6.BGI Res, Shenzhen 518083, Peoples R China

7.Qinghai Prov Peoples Hosp, Clin Res Inst Hydatid Dis, Xining 810007, Peoples R China

8.Jagiellonian Univ, Fac Biochem Biophys & Biotechnol, Lab Bioinformat & Genome Biol, Gronostajowa 7, PL-30387 Krakow, Poland

9.Sun Yat Sen Univ, Zhongshan Sch Med, Dept Immunol & Microbiol, Guangzhou 510080, Peoples R China

10.BGI Res, China Natl GeneBank, Shenzhen 518120, Peoples R China

11.BGI Res, Chongqing 401329, Peoples R China

12.Jinfeng Lab, JFL BGI STOmics Ctr, Chongqing 401329, Peoples R China

13.South China Univ Technol, Sch Med, Guangzhou 510006, Peoples R China

14.BGI Res, Shenzhen Key Lab Trans Biotechnol, Shenzhen 518083, Peoples R China

15.BGI Res, Guangdong Prov Key Lab Genome Read & Write, Shenzhen 518083, Peoples R China

16.BGI Res, Belgrade 11000, Serbia

关键词： Echinococcus multilocularis; macrophages; neutrophils; single-cell RNA sequencing; spatial transcriptomics

期刊名称：ADVANCED SCIENCE （ 影响因子：14.1； 五年影响因子：15.6 ）

ISSN：

年卷期： 2025 年 12 卷 18 期

页码：

收录情况： SCI

摘要： Alveolar echinococcosis (AE) is caused by the chronic infection of E. multilocularis, whose tumor-like growth can lead to high fatality if improperly treated. The early diagnosis of infection and the treatment of advanced AE remain challenging. Herein, bulk RNA-seq, scRNA-seq, and spatial transcriptomics technologies are integrated, to reveal the host immune response mechanism against E. multilocularis both spatially and chronologically, collecting mouse liver samples at multiple timepoints up to 15 months post infection. These results unveil an unprecedented high-resolution spatial atlas of the E. multilocularis infection foci and the functional roles of neutrophils, Spp1+ macrophages, and fibroblasts during disease progression. The heterogeneity of neutrophil and macrophage subpopulations are critical in both parasite-killing and the occurrence of immunosuppression during AE progression. These findings indicate the transition of parasite control strategy from "active killing" to "negative segregation" by the host, providing instructive insights into the treatment strategy for echinococcosis.