Flexible chemiresistor biosensor array using PDMS, cellulose paper and semiconducting single-walled carbon nanotubes for simultaneous detection of ochratoxin A, deoxynivalenol, and zearalenone
文献类型： 外文期刊
作者： He, Yue 1 ; Wang, Hui 1 ; Yu, Zhixue 1 ; Chen, Ruipeng 1 ; Zhou, Mengting 1 ; Guo, Yuming 2 ; Tang, Xiangfang 1 ; Xiong, Benhai 1 ;
作者机构： 1.Chinese Acad Agr Sci, State Key Lab Anim Nutr & Feeding, Inst Anim Sci, Beijing 100193, Peoples R China
2.China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China
关键词： Chemiresistor; Mycotoxins; Semiconducting single-walled carbon nano; tubes; Simultaneous detection; Cellulose paper
期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.1； 五年影响因子：4.7 ）
ISSN： 0026-265X
年卷期： 2025 年 213 卷
页码：
收录情况： SCI
摘要： Multiple mycotoxins in foodstuffs and feeds are hazards to human and animal health through direct cell damage, immune suppression, genotoxicity, endocrine disruption, and nervous system harm. Herein, a low-cost paperbased chemiresistor biosensor array was established for the simultaneous determination of ochratoxin A (OTA), deoxynivalenol (DON), and zearalenone (ZEN). To overcome the poor mechanical properties, PDMS was introduced to fabricate a triple-channel electrochemical biosensor on cellulose paper, which was applied the single-wall carbon nanotubes (SWCNTs) and semiconducting SWCNTs (s-SWCNTs) as the conductive electrodes and sensitive material. The coupling conditions of antibody proteins with s-SWCNTs were discussed in detail, which a method using only EDC as the activating agent was employed to immobilize three bovine serum albumin conjugates (OTA-BSA, DON-BSA, and ZEN-BSA). After exploring optimal conditions of the chemiresistor biosensor array, the limit of detections for OTA, DON, and ZEN are determined to be 0.26, 0.45, and 0.21 pg/mL, respectively. The biosensor array was applied to detect mycotoxins in corn, demonstrating it possesses potential value for simultaneously determining multiple targets.
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