您好,欢迎访问中国农业科学院 机构知识库!

Small RNA deep sequencing reveals full-length genome of Citrus yellow vein clearing virus in Chongqing, China

文献类型: 外文期刊

作者: Yu Yun-qi 1 ; Wu Qiong 1 ; Su Hua-nan 3 ; Wang Xue-feng 2 ; Cao Meng-ji 2 ; Zhou Chang-yong 2 ;

作者机构: 1.Southwest Univ, Coll Plant Protect, Chongqing 400715, Peoples R China

2.Chinese Acad Agr Sci, Southwest Univ, Natl Citrus Engn & Technol Res Ctr, Citrus Res Inst, Chongqing 400712, Peoples R China

3.Gannan Normal Univ, Natl Navel Orange Engn & Technol Res Ctr, Ganzhou 341000, Peoples R China

关键词: citrus;Citrus yellow vein clearing virus (CYVCV);small RNA deep sequencing

期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )

ISSN: 2095-3119

年卷期: 2017 年 16 卷 2 期

页码:

收录情况: SCI

摘要: To identity the potential pathogen associated with the yellow vein clearing symptom on lemon trees, the profiles of virus-derived small interfering RNAs from citrus samples were obtained and analyzed by deep sequencing method in this study. Twenty-severi contigs almost cover the full length genome of Citrus yellow vein clearing virus (CYVCV) isolate YN were obtained using the small RNA deep sequencing technology. Analysis showed that this isolate CQ shared the highest nucleotide identity with isolate Y1 (JX040635) and YN (KP313242), both of which belong to the genus Mandarivirus in the family Alphaflexiviridae. Mapping analysis of viral-derived siRNA (vsiRNA) profiles revealed an uneven distribution pattern of their generations along both positive and negative genome strands, and 22- and 21-nt vsiRNAs ranked the majority. BLAST against viroids and other viral databases confirmed that this sample was single-infected only by CYVCV, which indicated that CYVCV was the exact causal agent for the yellow clearing symptom occurring on lemon. This is the first CYVCV isolate detected in Chongqing and the second in China. This result could provide a molecular basis for the investigation of citrus viral diseases to-protect citrus health in this region.

  • 相关文献

[1]中国葡萄类病毒基因多样性分析及其新种的发现. 姜冬梅,郭瑞,彭山,吴祖建. 2009

[2]中国栽培葡萄上发生的类病毒的种类鉴定. 王晶,SanoTeruo. 2003

[3]Mycorrhizal and Non-mycorrhizal Responses to Salt Stress in Trifoliate Orange: Plant Growth, Root Architecture and Soluble Sugar Accumulation. Zou, Ying-Ning,Wu, Qiang-Sheng,Liang, Yong-Chao,Wu, Qiang-Sheng. 2013

[4]The Mining of Citrus EST-SNP and Its Application in Cultivar Discrimination. Jiang Dong,Ye Qing-liang,Wang Fu-sheng,Cao Li. 2010

[5]Mycorrhizal symbiosis enhances tolerance to NaCl stress through selective absorption but not selective transport of K+ over Na+ in trifoliate orange. Wu, Qiang-Sheng,Zou, Ying-Ning,He, Xin-Hua,He, Xin-Hua. 2013

[6]Cryopreservation of Citrus anthers in the National Crop Genebank of China. Zhang, Jin-Mei,Lu, Xin-Xiong,Xin, Xia,Yin, Guang-Kun,He, Juan-Juan,Huang, Bin,Chen, Xiao-Ling,Huang, Bin,Jiang, Dong. 2017

[7]Genomic characterization of miR156 and SQUAMOSA promoter binding protein-like genes in sweet orange (Citrus sinensis). Liu, Mei-Ya,Wu, Xiao-Meng,Long, Jian-Mei,Guo, Wen-Wu,Liu, Mei-Ya.

[8]Identification of microRNAs correlated with citrus granulation based on bioinformatics and molecular biology analysis. Zhang, Jing,Wang, Miao,Dai, Chao,Sun, Yufeng,Lu, Jia,Huang, Yatao,Li, Minmin,He, Yan,Wang, Fengzhong,Fan, Bei,Zhang, Jing,Wang, Miao,Dai, Chao,Sun, Yufeng,Lu, Jia,Huang, Yatao,Li, Minmin,He, Yan,Wang, Fengzhong,Fan, Bei,Cheng, Fansheng.

[9]Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection. Zou, Xiuping,Peng, Aihong,Xu, Lanzhen,Liu, Xiaofeng,Lei, Tiangang,Yao, Lixiao,He, Yongrui,Chen, Shanchun,Zou, Xiuping,Peng, Aihong,Xu, Lanzhen,Liu, Xiaofeng,Lei, Tiangang,Yao, Lixiao,He, Yongrui,Chen, Shanchun. 2013

[10]Obtaining citrus hybrids by in vitro culture of embryos from mature seeds and early identification of hybrid seedlings by allele-specific PCR. Zhu, Shiping,Zhong, Guangyan,Wu, Bo,Ma, Yanyan,Chen, Jiao,Zhong, Guangyan. 2013

[11]Behaviour of spirotetramat residues and its four metabolites in citrus marmalade during home processing. Liu, Yanyu,Chen, Weijun,Sun, Dali,Gong, Lei,Jiang, Liyan,Jiao, Bining,Liu, Yanyu,Chen, Weijun,Sun, Dali,Gong, Lei,Jiang, Liyan,Jiao, Bining,Su, Xuesu,Jian, Qiu,Chen, Weijun,Jiao, Bining,Jiao, Bining.

[12]The ARF, AUX/IAA and GH3 gene families in citrus: genome-wide identification and expression analysis during fruitlet drop from abscission zone A. Xie, Rangjin,Pang, Shaoping,Ma, Yanyan,Deng, Lie,He, Shaolan,Yi, Shilai,Lv, Qiang,Zheng, Yongqiang.

[13]A deep-coverage BAC library for Ponkan mandarin and its further use in screening ethylene regulated genes. Wang, Miao,Tan, AnQun,Zhang, LingYun,Yang, JiaWei,Wei, ZhaoXin,Cheng, ChunZhen,Zhong, GuangYan,Zhou, ZhiQin,Zhong, GuangYan,Wang, Miao,Zhang, LingYun,Yang, JiaWei,Wei, ZhaoXin,Cheng, ChunZhen,Zhou, ZhiQin. 2010

[14]Genome-Wide Identification, Cloning and Functional Analysis of the Zinc/Iron-Regulated Transporter-Like Protein (ZIP) Gene Family in Trifoliate Orange (Poncirus trifoliata L. Raf.). Fu, Xing-Zheng,Zhou, Xue,Xing, Fei,Ling, Li-Li,Chun, Chang-Pin,Cao, Li,Peng, Liang-Zhi,Fu, Xing-Zheng,Zhou, Xue,Ling, Li-Li,Chun, Chang-Pin,Cao, Li,Peng, Liang-Zhi,Aarts, Mark G. M.. 2017

[15]The chemical components of essential oils from the leaves of 110 species and cultivars of Citrus plants. Huang, YZ,Chen, QY. 1998

[16]Characteristics and oleocellosis sensitivity of citrus fruits. Deng, Lie,Zheng, Yongqiang,Zhou, Zhiqin,Mao, Shasha,Zhao, Xuyang. 2010

[17]Genome-wide identification of sweet orange (Citrus sinensis) metal tolerance proteins and analysis of their expression patterns under zinc, manganese, copper, and cadmium toxicity. Fu, Xing-Zheng,Tong, Ya-Hua,Zhou, Xue,Ling, Li-Li,Chun, Chang-Pin,Cao, Li,Peng, Liang-Zhi,Tong, Ya-Hua,Zeng, Ming,Fu, Xing-Zheng,Zhou, Xue,Ling, Li-Li,Chun, Chang-Pin,Cao, Li,Peng, Liang-Zhi. 2017

[18]Production and evaluation of transgenic sweet orange (Citrus sinensis Osbeck) containing bivalent antibacterial peptide genes (Shiva A and Cecropin B) via a novel Agrobacterium-mediated transformation of mature axillary buds. He, Yongrui,Chen, Shanchun,Peng, Aihong,Zou, Xiuping,Xu, Lanzheng,Lei, Tiangang,Liu, Xiaofeng,Yao, Lixiao,He, Yongrui,Chen, Shanchun,Peng, Aihong,Zou, Xiuping,Xu, Lanzheng,Lei, Tiangang,Liu, Xiaofeng,Yao, Lixiao,He, Yongrui,Chen, Shanchun,Peng, Aihong,Zou, Xiuping,Xu, Lanzheng,Lei, Tiangang,Liu, Xiaofeng,Yao, Lixiao. 2011

[19]Comprehensive analysis of SAUR gene family in citrus and its transcriptional correlation with fruitlet drop from abscission zone A. Xie, Rangjin,Dong, Cuicui,Ma, Yanyan,Deng, Lie,He, Shaolan,Yi, Shilai,Lv, Qiang,Zheng, Yongqiang.

[20]Identification of fruit and branch in natural scenes for citrus harvesting robot using machine vision and support vector machine. Qiang, Lu,Lie, Deng,Cai Jianrong,Bin, Liu,Zhang Yajing. 2014

作者其他论文 更多>>