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Identification, characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.)

文献类型： 外文期刊

作者： Liu Wen-wen 1 ; Xin Min 1 ; Cao Meng-ji 2 ; Qin Meng 3 ; Liu Hui 3 ; Zhao Shou-qi 3 ; Wang Xi-feng 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

2.Southwest Univ, Citrus Res Inst, Natl Citrus Engn Res Ctr, Chongqing 400712, Peoples R China

3.Natl Agr Technol Extens & Serv Ctr, Beijing 100125, Peoples R China

关键词： common sunflower (Helianthus annuus); next-generation sequencing (NGS); double-stranded RNA; Endornaviridae

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2018 年 17 卷 10 期

页码：

收录情况： SCI

摘要： To identify the possible quarantine viruses in seven common sunflower varieties imported from the United States of America and the Netherlands, we tested total RNAs extracted from the leaf tissues using next-generation sequencing of small RNAs. After analysis of small RNA sequencing data, no any quarantine virus was found, but a double-stranded RNA (dsRNA) molecule showing typical genomic features of endornavirus was detected in two varieties, X3939 and SH1108. Full-length sequence and phylogenetic analysis showed that it is a novel endornavirus, temporarily named as Helianthus annuus alphaendornavirus (HaEV). Its full genome corresponds to a 14 662-bp dsRNA segment, including a 21-nt 5' untranslated region (UTR), 3'UTR ending with the unique sequence CCCCCCCC and lacking a poly(A) tail. An open reading frame (ORF) that encodes a deduced 4 867 amino acids (aa) polyprotein with three domains: RdRP, Hel and UGT (UDP-glycosyltransferase). HaEV mainly distributed in the cytoplasm but less in the nucleus of leaf cells by fluorescence in situ hybridization (FISH) experiment. This virus has a high seed infection rate in the five varieties, X3907, X3939, A231, SH1108 and SR1320. To our knowledge, this is the first report about the virus of the family Endornaviridae in the common sunflower.

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