Development of a sensitive and reliable droplet digital PCR assay for the detection of 'Candidatus Liberibacter asiaticus'
文献类型： 外文期刊
作者：
Zhong Xi
1
;
Liu Xue-lu
1
;
Lou Bing-hai
2
;
Zhou Chang-yong
1
;
Wang Xue-feng
1
;
作者机构： 1.Southwest Univ, Natl Citrus Engn Res Ctr, Citrus Res Inst, Chinese Acad Agr Sci, Chongqing 400712, Peoples R China
2.Guangxi Acad Specialty Crops, Guangxi Key Lab Citrus Biol, Guilin 541004, Peoples R China
关键词： citrus Huanglongbing;early diagnosis;droplet digital PCR
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）
ISSN： 2095-3119
年卷期： 2018 年 17 卷 2 期
页码：
收录情况： SCI
摘要： Citrus Huanglongbing (HLB, yellow shoot disease) is one of the most serious citrus diseases worldwide. To better improve the detection sensitivity, a droplet digital PCR (ddPCR) assay was developed for the rapid detection of 'Candidatus Liberibacter asiaticus' (Las), the putative causal agent of HLB. The detection of sensitivity comparison using positive plasmid indicated that ddPCR was superior to quantitative PCR (qPCR) for detecting and quantifying Las at low concentrations. The Las detection of 40 field samples also showed that six of 13 asymptomatic samples (46.15%) with high C-t value (> 35) were positive by ddPCR. This methodology showed great potential for early HLB infection diagnosis.
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