An Inter-Subspecies Cloned Buffalo (Bubalus bubalis) Obtained by Transferring of Cryopreserved Embryos via Somatic Cell Nuclear Transfer
文献类型： 外文期刊
作者： Yang, B. Z. 1 ; Yang, C. Y. 1 ; Li, R. C. 1 ; Qin, G. S. 1 ; Zhang, X. F. 1 ; Pang, C. Y. 1 ; Chen, M. T. 1 ; Huang, F. X 1 ;
作者机构： 1.Chinese Acad Agr Sci, Guangxi Buffalo Res Inst, Nanning 530001, Peoples R China
期刊名称：REPRODUCTION IN DOMESTIC ANIMALS （ 影响因子：2.005； 五年影响因子：2.154 ）
ISSN： 0936-6768
年卷期： 2010 年 45 卷 5 期
页码：
收录情况： SCI
摘要： The aim of this study was to explore the feasibility of cryopreservation of inter-subspecies cloned embryos in buffalo. In our experiment, river buffalo ear fibroblast nucleus was fused into swamp buffalo oocyte cytoplasm. The blastocyst formation rate for nuclear transfer of freshly thawed cells was not different from those of growing cells, confluent or serum-starved cells. A total of 122 cloned blastocysts derived from cryopreserved fibroblasts were cryopreserved and thawed, 37 were survived, the cryosurvival rate was 30.3%. The survived blastocysts were transferred into 15 recipient buffalos. Five of the recipients established pregnancy, but four of them aborted on day 53, 59, 145 and 179 of gestation respectively. One crossbred buffalo (Murrah x Swamp buffalo (2n = 49) received three embryos delivered a 40.5 kg female calf by natural delivery on day 320 of gestation. Up to now (13-month old), the cloned calf has been growing well with no abnormity observed. These results demonstrated that cryopreservation of inter-subspecies cloned embryos is feasible to produce buffalo offspring.
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