文献类型： 外文期刊

作者： Najeeb, S. ¹ ; Ali, J. ¹ ; Mahender, A. ¹ ; Pang, Y. L. ³ ; Zilhas, J. ¹ ; Murugaiyan, V ¹ ; Vemireddy, Lakshminarayan ¹ ;

作者机构： 1.Int Rice Res Inst, Rice Breeding Platform, Los Banos 4031, Laguna, Philippines

2.Sher E Kashmir Univ Agr Sci & Technol, Mt Res Ctr Field Crops, Khudwani 190025, Kashmir, India

3.Shandong Agr Univ, Coll Agron, State Key Lab Crop Biol, Tai An 271018, Peoples R China

4.Univ Bonn, Inst Crop Sci & Resource Conservat INRES, Plant Nutr, D-53012 Bonn, Germany

5.Acharya NG Ranga Agr Univ, Sri Venkateswara Agr Coll, Dept Genet & Plant Breeding, Tirupati 517502, Andhra Pradesh, India

6.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

关键词： Low-temperature stress; Germination; Early seedling growth stage; Stress index; SNP markers; Quantitative trait loci

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2020 年 40 卷 1 期

页码：

收录情况： SCI

摘要： An attempt was made in the current study to identify the main-effect and co-localized quantitative trait loci (QTLs) for germination and early seedling growth traits under low-temperature stress (LTS) conditions in rice. The plant material used in this study was an early backcross population of 230 introgression lines (ILs) in BCIF7 generation derived from the Weed Tolerant Rice-1 (WTR-1) (as the recipient) and Haoannong (HNG) (as the donor). Genetic analyses of LTS tolerance revealed a total of 27 main-effect quantitative trait loci (M-QTLs) mapped on 12 chromosomes. These QTLs explained more than 10% of phenotypic variance (PV), and average PV of 12.71% while employing 704 high-quality SNP markers. Of these 27 QTLs distributed on 12 chromosomes, 11 were associated with low-temperature germination (LTG), nine with low-temperature germination stress index (LTGS), five with root length stress index (RLSI), and two with biomass stress index (BMSI) QTLs, shoot length stress index (SLSI) and root length stress index (RLSI), seven with seed vigor index (SVI), and single QTL with root length (RL). Among them, five significant major QTLs (qLTG(I)(1), qLTGS(I)(1-2), qLTG(I)(5), qLTGS(I)(5), and qLTG(I)(7)) mapped on chromosomes 1, 5, and 7 were associated with LTG and LTGS traits and the PV explained ranged from 16 to 23.3%. The genomic regions of these QTLs were co-localized with two to six QTLs. Most of the QTLs were growth stage-specific and found to harbor QTLs governing multiple traits. Eight chromosomes had more than four QTLs and were clustered together and designated as promising LTS tolerance QTLs (qLTTs), as qLTT(1), qLTT(2), qLTT(3), qLTT(5), qLTT(6), qLTT(8), qLTT(9), and qLTT(11). A total of 16 putative candidate genes were identified in the major M-QTLs and co-localized QTL regions distributed on different chromosomes. Overall, these significant genomic regions of M-QTLs are responsible for multiple traits and this suggested that these could serve as the best predictors of LTS tolerance at germination and early seedling growth stages. Furthermore, it is necessary to fine-map these regions and to find functional markers for marker-assisted selection in rice breeding programs for cold tolerance.