您好,欢迎访问中国农业科学院 机构知识库!

Attenuation of Virulent Porcine Reproductive and Respiratory Syndrome Virus Strain CH-1a and Genetic Variation of ORF5 Gene

文献类型: 外文期刊

作者: Cai Xue-hui 1 ; Wu Guo-jun 2 ; Liu Yong-gang 2 ; Liu Guang-qing 3 ; Shi Wen-da 2 ; Wang Shu-jie 2 ; Ma Ping 2 ; Li Cheng- 1 ;

作者机构: 1.Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Peoples R China

2.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China

3.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Div Avian Infect Dis, Shanghai 200241, Peoples R China

关键词: attenuation;porcine reproductive and respiratory syndrome virus;genetic variation;ORF5 gene

期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )

ISSN: 2095-3119

年卷期: 2012 年 11 卷 12 期

页码:

收录情况: SCI

摘要: To develop a modified live vaccine (MLV) against porcine reproductive and respiratory syndrome virus (PRRSV), virulent CH-1a strain was attenuated by serial passages up to 130 passage (P130) in Marc-145 cells. The virulence and immune efficacy of the attenuated CH-1a were evaluated in pigs. The results showed that animals inoculated with P130 did not develop any clinical sign of the disease, but produced rapid and effective humoral immune responses against PRRSV challenge, indicating that attenuated CH-1a P130 is the candidate as the effective vaccine against PRRSV. To define the potential mutations in the attenuated CH-1a genome, we sequenced and analyzed the ORF5 gene of CH-1a strain of different passages (P39, P55, P65, P70, P85, P100, P115, P120, P125, and P130) and found that three mutations (C5Y, H38Q and L146Q) which may be related with the attenuation of CH-1a. In addition, we also found a unique restriction enzyme site (TspEI) in the ORF5 gene of attenuated CH-1a, which can be used as a genetic marker to distinguish original and attenuated CH-1a.

  • 相关文献

[1]Mutations in the genome of the highly pathogenic porcine reproductive and respiratory syndrome virus potentially related to attenuation. Leng, Xue,Li, Zhenguang,Wang, Fengxue,Wang, Wei,Zhang, Xiuhua,Wu, Hua,Xia, Mingqi,Li, Xiyu,Wu, Hua.

[2]Automated Measurement of Pericoronary Adipose Tissue Attenuation and Volume in Ct Angiography. Andrew M. Nguyen,Tejas Sudharshan Mathai,Liangchen Liu,Jianfei Liu,Ronald M. Summers. 2024

[3]Comparative Genomics of Mycoplasma bovis Strains Reveals That Decreased Virulence with Increasing Passages Might Correlate with Potential Virulence-Related Factors. Rasheed, Muhammad A.,Qi, Jingjing,Zhu, Xifang,He Chenfei,Menghwar, Harish,Khan, Farhan A.,Gang Zhao,Zubair, Muhammad,Chen, Huanchun,Guo, Aizhen,Rasheed, Muhammad A.,Zhu, Xifang,He Chenfei,Menghwar, Harish,Khan, Farhan A.,Gang Zhao,Zubair, Muhammad,Hu, Changmin,Chen, Yingyu,Chen, Huanchun,Guo, Aizhen,Rasheed, Muhammad A.,Qi, Jingjing,Chen, Huanchun,Guo, Aizhen,Chen, Huanchun,Guo, Aizhen. 2017

[4]Attenuation of an original US porcine epidemic diarrhea virus strain PC22A via serial cell culture passage. Lin, Chun-Ming,Hou, Yixuan,Gao, Xiang,Liu, Xinsheng,Zheng, Lanlan,Saif, Linda J.,Wang, Qiuhong,Marthaler, Douglas G.,Marthaler, Douglas G.,Liu, Xinsheng,Zheng, Lanlan.

[5]Live attenuated pseudorabies virus developed using the CRISPR/Cas9 system. Tang, Yan-Dong,Liu, Ji-Ting,Wang, Tong-Yun,An, Tong-Qing,Sun, Ming-Xia,Wang, Shu-Jie,Fang, Qiong-Qiong,Hou, Lin-lin,Tian, Zhi-Jun,Cai, Xue-Hui,Liu, Ji-Ting.

[6]Development of a live attenuated vaccine candidate against duck Tembusu viral disease. Li, Guoxin,Gao, Xuyuan,Xiao, Yali,Liu, Shaoqiong,Peng, Shan,Li, Xuesong,Shi, Ying,Zhang, Yuee,Yu, Lei,Wu, Xiaogang,Yan, Pixi,Yan, Liping,Teng, Qiaoyang,Tong, Guangzhi,Li, Zejun. 2014

[7]Complete genome comparison of duck hepatitis virus type 1 parental and attenuated strains. Wang, Yong,Li, Chuanfeng,Chen, Zongyan,Li, Gang,Wang, Yong,Xu, Binrui,Li, Gang.

[8]Genetic variation in the long terminal repeat associated with the transition of Chinese equine infectious anemia virus from virulence to avirulence. Wei, Lili,Lu, Xiaoling,Zhao, Liping,Xiang, Wenhua,Xue, Fei,Shen, Rongxian,Wang, Xiaojun,Wei, Lili,Fan, Xiujuan,Zhang, Xiaoyan,Shao, Yiming. 2009

[9]Cloning of the genome of a goose parvovirus vaccine strain SYG61v and rescue of infectious virions from recombinant plasmid in embryonated goose eggs. Wang, Jianye,Duan, Jinkun,Meng, Xia,Jiang, Zhiwei,Zhu, Guoqiang,Wang, Jianye,Duan, Jinkun,Meng, Xia,Jiang, Zhiwei,Zhu, Guoqiang,Gong, Jiansen. 2014

[10]Molecular characterization of a Chinese vaccine strain of transmissible gastroenteritis virus: mutations that may contribute to attenuation. Wang, Chengbao,Chen, Jianfei,Shi, Hongyan,Qiu, Huaji,Xue, Fei,Liu, Changming,Zhu, Yuanmao,Liu, Shengwang,Feng, Li,Almazan, Fernando,Enjuanes, Luis. 2010

[11]Changes in VP3 and VP5 genes during the attenuation of the very virulent infectious bursal disease virus strain Gx isolated in China. Wang, Xiaomei,Zhang, Houshuang,Gao, Honglei,Fu, Chaoyang,Gao, Yulong,Ju, Yulin. 2007

[12]Mechanisms of suppression of interferon production by porcine reproductive and respiratory syndrome virus. Zhu, L.,Zhou, Y.,Tong, G.. 2012

[13]Development and validation of reverse transcription loop-mediated isothermal amplification for detection of PRRSV. Chen, Changmu,Cui, Shangjin,Zhang, Chaofan,Li, Jun,Wang, Jinbao. 2010

[14]Development and validation of an immunogold chromatographic test for on-farm detection of PRRSV. Zhou, Sheng-hua,Cui, Shang-jin,Chen, Chang-mu,Zhang, Fan-chao,Li, Jun,Zhou, Shun.

[15]Antiviral activity of phage display selected peptides against Porcine reproductive and respiratory syndrome virus in vitro. Liu, Ke,Feng, Xiuli,Zhou, Bin,Cao, Ruibing,Huang, Li,Pang, Ran,He, Danni,Lian, Xue,Chen, Puyan,Ma, Zhiyong,Luo, Chao,Miao, Denian.

[16]Proteomic Analysis of the Secretome of Porcine Alveolar Macrophages Infected with Porcine Reproductive and Respiratory Syndrome Virus. Li, Yuming,Wu, Zhuanchang,Liu, Ke,Qi, Pengfei,Xu, Jinpeng,Wei, Jianchao,Li, Beibei,Shao, Donghua,Shi, Yuanyuan,Qiu, Yafeng,Ma, Zhiyong.

[17]Nonstructural Protein 4 of Porcine Reproductive and Respiratory Syndrome Virus Modulates Cell Surface Swine Leukocyte Antigen Class I Expression by Downregulating beta 2-Microglobulin Transcription. Qi, Pengfei,Liu, Ke,Wei, Jianchao,Li, Yuming,Li, Beibei,Shao, Donghua,Wu, Zhuanchang,Shi, Yuanyuan,Tong, Guangzhi,Qiu, Yafeng,Ma, Zhiyong.

[18]Isolation and Sequence Analysis of Highly Pathogenic Porcine reproductive and respiratory syndrome virus from Swine Herds in the Jilin Province of China. Wang, Feng-Xue,Guo, Li,Yang, Yan-Ling,Song, Ni,Chen, Li-Zhi,Cheng, Shi-Peng,Wen, Yong-Jun. 2013

[19]Non-structural protein 2 of the porcine reproductive and respiratory syndrome (PRRS) virus: A crucial protein in viral pathogenesis, immunity and diagnosis. Wang, Feng-Xue,Song, Ni,Chen, Li-Zhi,Cheng, Shi-Peng,Wen, Yong-Jun,Wu, Hua. 2013

[20]Generation and Immunogenicity of a Recombinant Adenovirus Co-Expressing the E2 Protein of Classical Swine Fever Virus and the GP5 Protein of Porcine Reproduction and Respiratory Syndrome Virus. Li Hong-yu,Sun Yuan,Zhang Xing-juan,Chang Tian-ming,Wang Xiang-peng,He Fan,Huang Jun-hua,Qiu Hua-ji. 2011

作者其他论文 更多>>