您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Insight into the surface discharge cold plasma efficient inactivation of Pseudomonas fluorescens in water based on exogenous reactive oxygen and nitrogen species: Synergistic mechanism and energy benefits

文献类型： 外文期刊

作者： Ni, Jia-Bao 1 ; Ding, Chang-Jiang 3 ; Zhang, Jing-Shou 1 ; Fang, Xiao-Ming 2 ; Xiao, Hong-Wei 1 ;

作者机构： 1.China Agr Univ, Coll Engn, POB 194,17 Qinghua Donglu, Beijing 100083, Peoples R China

2.Chinese Acad Agr Sci, Inst Apicultural Res, State Key Lab Resource Insects, 1 Xiangshan Beigou, Beijing 100093, Peoples R China

3.Inner Mongolia Univ Technol, Coll Sci, Hohhot, Peoples R China

关键词： Surface discharge cold plasma; Inactivation; Pseudomonas fluorescens; Energy efficiency; Genes

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：12.2； 五年影响因子：11.9 ）

ISSN： 0304-3894

年卷期： 2024 年 476 卷

页码：

收录情况： SCI

摘要： As well known, surface discharge cold plasma has efficient inactivation ability and a variety of RONS are main active particles for inactivation, but their synergistic mechanism is still not clear. Therefore, surface discharge cold plasma system was applied to treat Pseudomonas fluorescens to study bacterial inactivation mechanism and energy benefit. Results showed that energy efficiency was directly proportional to applied voltage and inversely proportional to initial concentration. Cold plasma treatment for 20 min was inactivated by approximately > 4-log(10) Pseudomonas fluorescens and application of center dot OH and O-1(2) scavengers significantly improved survival rate. In addition, center dot OH and O-1(2) destroyed cell membrane structure and membrane permeability, which promoted diffusion of RONS into cells and affecting energy metabolism and antioxidant capacity, leading to bacterial inactivation. Furthermore, accumulation of intracellular NO and ONOOH was related to infiltration of exogenous RNS, while accumulation of center dot OH, H2O2, O-1(2), O-2(-) was the result of joint action of endogenous and exogenous ROS. Transcriptome analysis revealed that different RONS of cold plasma were responsible for Pseudomonas fluorescens inactivation and related to activation of intracellular antioxidant defense system and regulation of genes expression related to amino acid metabolism and energy metabolism, which promoting cellular process, catalytic activity and other biochemical pathways.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台