文献类型： 外文期刊
作者： Wang, Xue 1 ; Gao, Yao-Yao 3 ; Wang, Dun 1 ; Zhang, Qi 2 ; Wang, Hao-Ran 1 ; Zhang, Ting-Ting 1 ; Zhu, Meng-Jie 1 ; Dong, Jing 1 ; Ling, Dong 1 ; Feng, Peng 1 ; Tang, Xue-Hui 1 ; Li, Pei-Wu 2 ;
作者机构： 1.Xiangyang Acad Agr Sci, Xiangyang 441057, Peoples R China
2.Hubei Hongshan Lab, Wuhan 430061, Peoples R China
3.Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Peoples R China
4.Minist Agr & Rural Affairs, Key Lab Biotoxin Detect, Wuhan 430062, Peoples R China
5.Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430062, Peoples R China
关键词： aflatoxin; helicase; peroxidase; biodegradation
期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）
ISSN：
年卷期： 2025 年 14 卷 6 期
页码：
收录情况： SCI
摘要： To address the issue of aflatoxin contamination, which poses a significant threat to food safety and human health, we have conducted extensive research. We have isolated a strain of Myroides odoratimimus (3J2MO) from the soil that exhibited remarkable efficiency in degrading various aflatoxin types, including AFB1, AFB2, AFG1, AFG2, and AFM1. SDS-PAGE analysis confirmed the purity of the enzymes to be over 95%. Through fluorescence assays, we quantified the enzymatic activity, with an AFB1 degradation rate of 95% achieved at 37 degrees C and a pH of 8.0. Further analysis using HPLC-MS/MS identified the degradation intermediates, revealing the mechanisms of lactone ring cleavage and epoxy group hydrolysis. GO/COG/KEGG annotations provided insights into the functions of these enzymes, with peroxidase linked to reactive oxygen species (ROS) generation and helicase associated with ATP-dependent conformational changes. Helicase, on the other hand, hydrolyzes ATP, driving conformational changes in AFB1 and facilitating its breakdown into non-toxic metabolites. The potential industrial-scale application of this discovery could significantly mitigate aflatoxin-related economic losses while minimizing chemical residues in the food chain.
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