Smartphone-assisted electrochemiluminescence imaging test strips towards dual-signal visualized and sensitive monitoring of aflatoxin B1 in corn samples
文献类型： 外文期刊
作者： Chen, Miao-Miao 1 ; Zhang, Min-Ling 5 ; Song, Xiao 5 ; Jiang, Jun 1 ; Tang, Xiaoqian 1 ; Zhang, Qi 1 ; Zhang, Xiuhua 5 ; Li, Peiwu 1 ;
作者机构： 1.Minist Agr & Rural Affairs, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Peoples R China
2.Chinese Acad Agr Sci, Oil Crops Res Inst, Qual Inspect & Test Ctr Oil seed Prod, Lab Risk Assessment Oilseed Prod Wuhan, Wuhan 430062, Peoples R China
3.Hubei Univ, Food Safety Res Inst, Wuhan 430062, Peoples R China
4.Hubei Hongshan Lab, Wuhan 430070, Peoples R China
5.Xianghu Lab, Hangzhou 311231, Peoples R China
6.Hubei Univ, Coll Chem & Chem Engn, Wuhan 430062, Peoples R China
关键词： Electrochemiluminescence imaging; Test strips; Nanomaterials; Dual-signal outputs; Aflatoxin B1; Food safety
期刊名称：CHINESE CHEMICAL LETTERS （ 影响因子：8.9； 五年影响因子：7.3 ）
ISSN： 1001-8417
年卷期： 2025 年 36 卷 1 期
页码：
收录情况： SCI
摘要： Aflatoxins B1 (AFB1) contamination in agro-food holds great threaten to human and animal health. Conventional test strips for rapid AFB1 visualized monitoring remains challenged by improvement of sensitivity and matrix interference resistance. In this case, we developed a portable electrochemiluminescence (ECL) imaging test strip with dual-signal outputs for AFB1 quantification in corn samples. RuPEI@SiO2 @Au nanospheres were synthesized for bonding with anti-AFB1 antibody and then colorimetrical signal-reported on test line through the capillary flow at strips. Meanwhile, ECL imaging signal of the constructed carbon-ink-based working electrode on polyvinyl chloride substrate of strips was exported under an applied potential of 1.25 V. The whole ECL test strips not only endowed convenient colorimetric responses but guaranteed quick-witted ECL image distinguishment even at extremely low AFB1 content. The detection limit of this ECL imaging-integrated mode was 10-fold lower than that of only colorimetric mode. Furthermore, satisfactory selectivity, reliability and practicability of the as-proposed ECL test strips were demonstrated. This work offered a promising platform for on-site, accurate and sensitive detection of pollutants in foods. (c) 2024 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
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