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Dual-channel microfluidic paper-based fluorescent immunosensor for simultaneous detection of aflatoxin B1 and capsaicin in edible oils

文献类型： 外文期刊

作者： Girmatsion, Mogos 1 ; Tang, Xiaoqian 1 ; Zhang, Qi 1 ; Wu, Wenqin 1 ; Hu, Xiaofeng 1 ; Li, Peiwu 1 ;

作者机构： 1.Oil Crops Res Inst, Chinese Acad Agr Sci, Wuhan 430062, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Biol & Genet Improvment Oil Crops, Wuhan, Peoples R China

3.Minist Agr & Rural Affairs, Lab Risk Assessment Oilseed Prod Wuhan, Wuhan, Peoples R China

4.Minist Agr & Rural Affairs, Qual Inspect & Test Ctr Oilseed Prod, Wuhan, Peoples R China

5.Hubei Univ, Food Safety Res Inst, Wuhan 430062, Peoples R China

6.Xianghu Lab, Hangzhou 311231, Peoples R China

7.Hubei Hongshan Lab, Wuhan 430070, Peoples R China

8.Hamelmalo Agr Coll, Dept Food Sci, Keren, Eritrea

关键词： Dual-channel paper device; Immunosensor; Phycocyanin; Aflatoxin B 1; Capsaicin; Edible oils

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.7； 五年影响因子：7.4 ）

ISSN：

年卷期： 2025 年 426 卷

页码：

收录情况： SCI

摘要： In this study, we developed a fluorescent immunosensor (FIS) based on a dual-channel microfluidic paper-based analytical device (mu PAD) for a concurrent detection of aflatoxin B1(AFB1) and capsaicin (CAP) in edible oils. The fluorescence-based detection was facilitated using Phycocyanin (PC) as a signal marker. PC, along with AFB1 or CAP-specific antibodies was successfully immobilized onto the surfaces of carboxyl-functionalized polystyrene latex nanospheres (LNS), ensuring stable attachment of the proteins. The binding of these immobilized antibodies to their corresponding antigens generated strong, detectable signals on the mu PAD following the flow of 35 mu L sample solution across the mu PAD channels. The signals were collected using a smartphone camera integrated with a 365 nm LED UV light source. The FIS-mu PAD system demonstrated enhanced sensitivity for both targets, achieving low detection limits of 0.23 ng/mL and 0.17 ng/mL for AFB1, and 0.63 ng/mL and 0.75 ng/mL CAP in soybean and corn oil samples, respectively. The accuracy of the method was thoroughly analyzed and cross- validated using standard technique. The new immunosensor, based on the dual-channel mu PAD offers an easy- to-use, rapid, cost-effective, and portable platform for the detection of AFB1 and CAP in edible oils.

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