Engineering plants using diverse CRISPR-associated proteins and deregulation of genome-edited crops
文献类型： 外文期刊
作者： Zaman, Qamar U. 1 ; Raza, Ali 5 ; Lozano-Juste, Jorge 6 ; Chao, Li 3 ; Jones, Michael G. K. 7 ; Wang, Hua-Feng 1 ; Varshney, Rajeev K. 7 ;
作者机构： 1.Hainan Univ, Sch Breeding & Multiplicat, Sanya Inst Breeding & Multiplicat, Sanya 572025, Peoples R China
2.Hainan Univ, Collaborat Innovat Ctr Nanfan & High Efficiency Tr, Sch Trop Crops, Haikou 570228, Peoples R China
3.Chinese Acad Agr Sci, Key Lab Biol, Xudong 2nd Rd, Wuhan 430062, Peoples R China
4.Chinese Acad Agr Sci, Genet Res Inst, Xudong 2nd Rd, Wuhan 430062, Peoples R China
5.Shenzhen Univ, Coll Life Sci & Oceanog, Guangdong Key Lab Plant Epigenet, Shenzhen 518060, Peoples R China
6.Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Valencia 46022, Spain
7.Murdoch Univ, Ctr Crop & Food Innovat, State Agr Biotechnol Ctr, Perth, WA 6150, Australia
期刊名称：TRENDS IN BIOTECHNOLOGY （ 影响因子：17.3； 五年影响因子：18.1 ）
ISSN： 0167-7799
年卷期： 2024 年 42 卷 5 期
页码：
收录情况： SCI
摘要： The CRISPR/Cas system comprises RNA -guided nucleases, the target specificity of which is directed by Watson-Crick base pairing of target loci with single guide (sg)RNA to induce the desired edits. CRISPR-associated proteins and other engineered nucleases are opening new avenues of research in crops to induce heritable mutations. Here, we review the diversity of CRISPR-associated proteins and strategies to deregulate genome-edited (GEd) crops by considering them to be close to natural processes. This technology ensures yield without penalties, advances plant breeding, and guarantees manipulation of the genome for desirable traits. DNA -free and off -target -free GEd crops with defined characteristics can help to achieve sustainable global food security under a changing climate, but need alignment of international regulations to operate in existing supply chains.
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