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Integrative analyses reveal Bna-miR397a-BnaLAC2 as a potential modulator of low-temperature adaptability in Brassica napus L.

文献类型： 外文期刊

作者： Hussain, Muhammad Azhar 1 ; Huang, Yong 1 ; Luo, Dan 1 ; Mehmood, Sundas Saher 1 ; Raza, Ali 1 ; Zhang, Xuekun 2 ; Cheng, Yong 1 ; Cheng, Hongtao 1 ; Zou, Xiling 1 ; Ding, Xiaoyu 1 ; Zeng, Liu 1 ; Duan, Liu 3 ; Wu, Bian 4 ; Hu, Keming 3 ; Lv, Yan 1 ;

作者机构： 1.Chinese Acad Agr Sci CAAS, Oil Crops Res Inst, Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, Wuhan, Peoples R China

2.Yangtze Univ, Jingzhou, Peoples R China

3.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan, Peoples R China

4.Hubei Acad Agr Sci, Food Crops Inst, Wuhan, Peoples R China

5.Yangzhou Univ, Jiangsu Key Lab Crop Genet & Physiol, Jiangsu Key Lab Crop Genom & Mol Breeding, Key Lab Plant Funct Genom,Minist Educ,Jiangsu Key, Yangzhou, Peoples R China

关键词： B. napus; cold stress; Bna-miR397a; BnaLAC2; lignin; ROS homeostasis

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：10.5； 五年影响因子：12.4 ）

ISSN： 1467-7644

年卷期： 2025 年 23 卷 6 期

页码：

收录情况： SCI

摘要： Brassica napus L. (B. napus) is a major edible oil crop grown around the southern part of China, which often faces cold stress, posing potential damage to vegetative tissues. To sustain growth and reproduction, a detailed understanding of fundamental regulatory processes in B. napus against long-term low temperature (LT) stress is necessary for breeders to adjust the level of LT adaption in a given region and is therefore of great economic importance. Till now, studies on microRNAs (miRNAs) in coping with LT adaption in B. napus are limited. Here, we performed an in-depth analysis on two B. napus varieties with distinct adaptability to LT stress. Through integration of RNA sequencing (RNA-seq) and small RNA-sequencing (sRNA-seq), we identified 106 modules comprising differentially expressed miRNAs and corresponding potential targets based on strong negative correlations between their dynamic expression patterns. Specifically, we demonstrated that Bna-miR397a post-transcriptionally regulates a LACCASE (LAC) gene, BnaLAC2, to enhance the adaption to LT stresses in B. napus by reducing the total lignin remodelling and ROS homeostasis. In addition, the miR397-LAC2 module was also proved to improve freezing tolerance of Arabidopsis, indicating a conserved role of miR397-LAC2 in Cruciferae plants. Overall, this work provides the first description of a miRNA-mediated-module signature for LT adaption and highlights the prominent role of laccase in future breeding programme of LT tolerant B. napus.

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