您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular characterisation and developmental expression analysis of the 5-HT7 receptor in Chrysopa formosa

文献类型： 外文期刊

作者： Li, Fei 1 ; Wang, Longrui 2 ; Li, Yujia 2 ; Tian, Xuyang 2 ; Wang, Jiayue 2 ; Shen, Zhongjian 2 ; Zhang, Junjie 1 ; Zhang, Lisheng 2 ; Li, Yuyan 2 ;

作者机构： 1.Jilin Agr Univ, Inst Biol Control, Engn Res Ctr Nat Enemies, Jilin Prov Int Cooperat Key Lab Biol Control Agr P, Changchun, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Key Lab Nat Enemy Insects,Minist Agr & Rural Affai, Beijing, Peoples R China

3.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Key Lab Anim Biosafety Risk Prevent & Control Nort, Minist Agr & Rural Affairs, Shanghai, Peoples R China

关键词： 5-HT7 receptor; diapause; expression pattern; lacewing; serotonin

期刊名称：BULLETIN OF ENTOMOLOGICAL RESEARCH （ 影响因子：1.6； 五年影响因子：1.7 ）

ISSN： 0007-4853

年卷期： 2025 年 115 卷 3 期

页码：

收录情况： SCI

摘要： Serotonin (5-hydroxytryptamine, 5-HT) is a key monoamine neurotransmitter in insects, which regulates neural functions and influences various developmental and physiological processes by binding to its receptors. In this study, we investigate the molecular characteristics, phylogenetic relationships, and expression patterns of the 5-HT7 receptor (Cf5-HT7) in Chrysopa formosa, with a focus on its potential involvement in developmental and diapause regulation. The Cf5-HT7 gene was identified and cloned from the C. formosa transcriptome, revealing an open reading frame of 1788 bp encoding a 596 amino acid protein. Sequence analysis confirmed that Cf5-HT7 is a typical class A G protein-coupled receptor, characterised by seven transmembrane domains and several post-translational modifications, including palmitoylation and N-glycosylation sites. Phylogenetic analysis revealed that Cf5-HT7 is most closely related to the 5-HT7 receptor from Chrysoperla carnea, with high conservation of key motifs involved in ligand binding and receptor activation. Expression analysis across different developmental stages of C. formosa showed that Cf5-HT7 is highly expressed in the first instar larvae, with significant upregulation observed during the prepupal stage. Under diapause-inducing conditions, Cf5-HT7 expression is modulated in a stage-specific manner, showing a marked decrease at the onset of diapause, followed by a significant increase during the mid-to-late diapause maintenance phase. These findings suggest that it plays a pivotal role in regulating development and diapause processes in C. formosa, offering new insights into the molecular mechanisms governing insect life cycle transitions. This study lays the groundwork for future research into the functional roles of 5-HT7 receptors in insect physiology and their potential applications in manipulating diapause.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台