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Development of a Noninfectious Japanese Encephalitis Virus Replicon for Antiviral Drug Screening and Gene Function Studies

文献类型： 外文期刊

作者： Yang, Yang 1 ; Zheng, Jiayang 1 ; Lin, Yafang 1 ; Zhang, Yan 2 ; Zhao, Qianming 3 ; Zhang, Hailong 1 ; Zhang, Junjie 1 ; Li, Zongjie 1 ; Liu, Ke 1 ; Li, Beibei 1 ; Shao, Donghua 1 ; Qiu, Yafeng 1 ; Ma, Zhiyong 1 ; Wei, Jianchao 1 ;

作者机构： 1.Chinese Acad Agr Sci, Shanghai Inst Infect Dis & Biosecur, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China

2.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China

3.Henan Agr Univ, Coll Vet Med, Zhengzhou 450046, Peoples R China

关键词： Japanese encephalitis virus; replicon; green fluorescent protein; antiviral drug screening; RNA replication

期刊名称：VIRUSES-BASEL （ 影响因子：3.5； 五年影响因子：3.7 ）

ISSN：

年卷期： 2025 年 17 卷 6 期

页码：

收录情况： SCI

摘要： Viral replicons are efficient tools to understand the mechanisms of viral replication and screen antiviral drugs. In this study, a viral-cDNA-based replicon of Japanese encephalitis virus (JEV), which is the causative agent of Japanese encephalitis, was constructed by replacing the viral structural proteins with a green fluorescent protein (JEV-GFP replicon). The resulting JEV-GFP replicon was used as a tool to screen antiviral drugs targeting JEV nonstructural proteins, and the five compounds JNJ-A07, HZ-1157, NITD-2, quinine, and NITD008 were obtained, which significantly inhibited the replication of the JEV-GFP replicon and JEV in vitro, and the properties of these five compounds were also analyzed. The CC50, EC50, and SI indices of these five compounds were analyzed. In addition, the JEV-GFP replicon was used as a tool to identify the residues of viral nonstructural proteins involved in RNA replication, and the cysteine residue at position 4 of nonstructural protein 1 was found to be essential for JEV RNA replication. These data suggested that the noninfectious JEV-GFP replicon could be used as tool for different purposes, such as antiviral drug screening and gene function studies.

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