Picoxystrobin causes mitochondrial dysfunction in earthworms by interfering with complex enzyme activity and binding to the electron carrier cytochrome c protein
文献类型： 外文期刊
作者： Zhao, Wentian 1 ; Wang, Kai 2 ; Mu, Xiyan 3 ; Jiang, Jiazhen 1 ; Yang, Yang 4 ; Wang, Chengju 1 ;
作者机构： 1.China Agr Univ, Coll Sci, Innovat Ctr Pesticide Res, Dept Appl Chem, Yuanming Yuan West Rd 2, Beijing 100193, Peoples R China
2.Shenyang Agr Univ, Coll Plant Protect, 120 Dongling Rd, Shenyang, Peoples R China
3.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China
4.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China
关键词： Picoxystrobin; Earthworm; Mitochondrial dysfunction; Environmental level; Molecular docking
期刊名称：ENVIRONMENTAL POLLUTION （ 影响因子：7.3； 五年影响因子：8.1 ）
ISSN： 0269-7491
年卷期： 2025 年 368 卷
页码：
收录情况： SCI
摘要： Picoxystrobin (PICO) poses a great threat to earthworms due to its widespread use in agriculture and its stability in soil. Mitochondria may be a sensitive target organ for the toxic effects of PICO on worms. Therefore, evaluating the effect of PICO on mitochondria can further understand the toxic mechanism of PICO to earthworms. Here, we investigated the mechanism of mitochondrial toxicity of PICO to earthworms at environmentally relevant concentrations (62.5, 125 and 250 mu g/kg). Transmission electron microscopy observed that PICO disrupted mitochondrial ultrastructure. PICO reduced mitochondrial membrane potential and inhibited the expression of mitochondrial dynamics proteins of 51 kDa (MiD51). PICO interfered with the electron transport chain (ETC) complex activity and the relative transcription of its subunits. In particular, PICO inhibited complex III activity, cytochrome c (Cyt c, an electron carrier protein that transfers electrons from complex III to complex IV) activity, and ATP level. These changes were observed at PICO concentrations as low as 62.5 mu g/kg. Molecular docking analysis indicated that PICO can directly bind to Cyt c with a minimum free energy of -7.84 kcal/mol, thus hindering electron transfer in the ETC. This study confirmed that PICO induces mitochondrial dysfunction in earthworms and contributes to effective pesticide management.
- 相关文献
作者其他论文 更多>>
-
Post-anthesis water use and biomass accumulation in winter wheat under subsoiling and microsprinkler irrigation
作者：Huang, Chao;Zhang, Yanyan;Liu, Xuchen;Gao, Yang;Ma, Shoutian;Qin, Anzhen;Li, Ying;Liu, Zhandong;Huang, Chao;Zhang, Yanyan;Liu, Xuchen;Gao, Yang;Ma, Shoutian;Qin, Anzhen;Li, Ying;Liu, Zhandong;Huang, Chao;Zhang, Yanyan;Liu, Xuchen;Ma, Shoutian;Qin, Anzhen;Li, Ying;Liu, Zhandong;Huang, Chao;Zhang, Qifeng;Gao, Zile;Wu, Guanghui;Wang, Kai
关键词：Microsprinkler irrigation; Winter wheat; Water consumption characteristics; Post-anthesis biomass accumulation; Grain-filling characteristics; North China Plain
-
Macro- and micro-plastic accumulation in soils under different intensive farming systems: A case study in Quzhou county, the North China Plain
作者：Zhang, Hanyue;Wang, Kai;Liu, Xuejun;Zhang, Hanyue;Yang, Xiaomei;Ritsema, Coen J.;Geissen, Violette;Yang, Xiaomei;Cui, Jixiao;Yan, Changrong;Yan, Changrong
关键词：Macro/micro-plastics; Intensive farming systems; Plastic film; North China Plain
-
MtCro: multi-task deep learning framework improves multi-trait genomic prediction of crops
作者：Chao, Dian;Wan, Fengqiang;Yang, Yang;Wang, Hao;Yan, Shen;Fang, Wei
关键词：Deep learning; Genomic prediction; Multi-task learning; Crop breeding
-
Broflanilide induces zebrafish neurobehavioral defects by interfering with synaptic homeostasis
作者：Wang, Kai;Liu, Xiaoyu;He, Lu;Mu, Xiyan;Wang, Chengju
关键词：broflanilide; Neural behavior changes; Synaptic injury; Neural cell heterogeneous response; Zebrafish
-
Genome-wide identification of peanut ERFs and functional characterization of AhERF28 in response to salt and drought stresses
作者：Tang, Haohong;Shi, Haonan;He, Zhaojie;Ma, Xiaohu;Wang, Kai;Liu, Feng;Cheng, Shuaishuai;Sun, Jie;Zhu, Qian-Hao;Xiong, Xianpeng
关键词：
Arachis hypogaea L.;ERF ; Abiotic stress;AhERF28 ; VIGS -
Insights into the role of GhCYP and GhTPS in the gossypol biosynthesis pathway via a multiomics and functional-based approach in cotton
作者：Mehari, Teame Gereziher;Fang, Hui;Wang, Kai;Yao, Dengbing;Wang, Baohua;Skoric, Marijana;Siler, Branislav;Liu, Fang;Araya, Tesfay
关键词：cotton; gossypol; near-isogenic lines; transcriptome; target metabolomics; correlation
-
Development of a Noninfectious Japanese Encephalitis Virus Replicon for Antiviral Drug Screening and Gene Function Studies
作者：Yang, Yang;Zheng, Jiayang;Lin, Yafang;Zhang, Hailong;Zhang, Junjie;Li, Zongjie;Liu, Ke;Li, Beibei;Shao, Donghua;Qiu, Yafeng;Ma, Zhiyong;Wei, Jianchao;Zhang, Yan;Zhao, Qianming
关键词：Japanese encephalitis virus; replicon; green fluorescent protein; antiviral drug screening; RNA replication
