Insights into the role of GhCYP and GhTPS in the gossypol biosynthesis pathway via a multiomics and functional-based approach in cotton
文献类型： 外文期刊
作者： Mehari, Teame Gereziher 1 ; Skoric, Marijana 2 ; Fang, Hui 1 ; Wang, Kai 1 ; Liu, Fang 3 ; Araya, Tesfay 4 ; Siler, Branislav 2 ; Yao, Dengbing 1 ; Wang, Baohua 1 ;
作者机构： 1.Nantong Univ, Sch Life Sci, Nantong 226019, Peoples R China
2.Univ Belgrade, Inst Biol Res Sinisa Stankov, Natl Inst Republ Serbia, Dept Plant Physiol, Belgrade 11108, Serbia
3.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biobreeding & Integrated Util, Anyang 455000, Peoples R China
4.Univ Free State, Dept Soil Crop & Climate Sci, POB 339, ZA-9300 Bloemfontein, South Africa
关键词： cotton; gossypol; near-isogenic lines; transcriptome; target metabolomics; correlation
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.4； 五年影响因子：4.8 ）
ISSN： 2095-3119
年卷期： 2025 年 24 卷 5 期
页码：
收录情况： SCI
摘要： Two cotton research institute (CRI) near-isogenic lines, CRI-12 glanded and CRI-12 glandless, were used to pinpoint potential genes and metabolic pathways linked to gossypol biosynthesis through transcriptome sequencing. We discovered more than 235 million clean reads and 1,184 differentially expressed genes (DEGs). Consecutively, we conducted a weighted gene co-expression network analysis and found a strong correlation between white and yellow modules containing GhTPS (GH_D09G0090) and GhCYP (GH_D05G2016) hub genes with the gossypol content. Importance of the GhTPS and GhCYP genes was demonstrated using RT-qPCR, virusinduced gene silencing (VIGS), and target metabolite analysis. Silencing these genes resulted in fewer glands on both leaves and stems two weeks after the infection compared to the wild type. In addition, 152 metabolites were identified through targeted metabolite profiling. Differential metabolite screening revealed 12 and 18 significantly different metabolites in TRV:GhTPS and TRV:GhCYP plants vs. the control group, respectively, showing a reduction in the accumulation of metabolites compared to the control. Content of hemigossypol, the final product of gossypol biosynthesis, was also reduced, as revealed by target metabolite analysis, suggesting the role of these genes in the gossypol biosynthetic pathway. Furthermore, a highly significant difference in gossypol content between the glanded and glandless lines was recorded. Findings of this study reveal a strong link between the gossypol content and GhTPS and GhCYP hub genes, suggesting their role in the gossypol biosynthetic pathway to reduce the accumulation of hemigossypol, which may offer new comprehension into the regulatory checkpoints of the gossypol biosynthesis pathway in cotton.
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