您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Maize transcription factor ZmEREB167 negatively regulates starch accumulation and kernel size

文献类型： 外文期刊

作者： Qing, Xiangyu 1 ; Li, Jianrui 2 ; Lin, Zhen 1 ; Wang, Wei 3 ; Yi, Fei 3 ; Chen, Jian 1 ; Liu, Qiujie 1 ; Song, Weibin 1 ; Lai, Jinsheng 1 ; Chen, Baojian 1 ; Zhao, Haiming 1 ; Yang, Zhijia 1 ;

作者机构： 1.China Agr Univ, Coll Agron & Biotechnol, Natl Maize Improvement Ctr, Dept Plant Genet & Breeding,State Key Lab Maize Bi, Beijing 100193, Peoples R China

2.Chinese Acad Agr Sci, Inst Crop Sci, State Key Lab Crop Gene Resources & Breeding, Beijing 100081, Peoples R China

3.China Agr Univ, Coll Agron & Biotechnol, Engn Res Ctr Plant Growth Regulator, Beijing 100193, Peoples R China

4.Frontiers Sci Ctr Mol Design Breeding, Beijing 100193, Peoples R China

5.China Agr Univ, Sanya Inst, Sanya 572024, Hainan, Peoples R China

关键词： Maize; EREB protein; BETL; Starch; Kernel development

期刊名称：JOURNAL OF GENETICS AND GENOMICS （ 影响因子：7.1； 五年影响因子：6.4 ）

ISSN： 1673-8527

年卷期： 2025 年 52 卷 3 期

页码：

收录情况： SCI

摘要： Transcription factors play critical roles in the regulation of gene expression during maize kernel development. The maize endosperm, a large storage organ, accounting for nearly 90% of the dry weight of mature kernels, serves as the primary site for starch storage. In this study, we identify an endosperm-specific EREB gene, ZmEREB167, which encodes a nucleus-localized EREB protein. Knockout of ZmEREB167 significantly increases kernel size and weight, as well as starch and protein content, compared with the wild type. In situ hybridization experiments show that ZmEREB167 is highly expressed in the BETL as well as PED regions of maize kernels. Dual-luciferase assays show that ZmEREB167 exhibits transcriptionally repressor activity in maize protoplasts. Transcriptome analysis reveals that a large number of genes are up-regulated in the Zmereb167-C1 mutant compared with the wild type, including key genetic factors such as ZmMRP-1 and ZmMN1, as well as multiple transporters involved in maize endosperm development. Integration of RNA-seq and ChIP-seq results identify 68 target genes modulated by ZmEREB167. We find that ZmEREB167 directly targets OPAQUE2, ZmNRT1.1, ZmIAA12, ZmIAA19, and ZmbZIP20, repressing their expressions. Our study demonstrates that ZmEREB167 functions as a negative regulator in maize endosperm development and affects starch accumulation and kernel size. Copyright (c) 2025, The Authors. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台