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Detection and characterization of bovine hepacivirus in cattle and sheep from Hulunbuir, northeastern China

文献类型： 外文期刊

作者： Ma, Jingge 1 ; Wei, Zhiwei 3 ; Li, Liang 4 ; Wang, Wei 5 ; Liu, Ziyan 1 ; Liu, Ning 1 ; Wei, Feng 2 ; Zheng, Xiangyu 3 ; Wang, Zedong 1 ;

作者机构： 1.First Hosp Jilin Univ, Ctr Infect Dis & Pathogen Biol, Dept Infect Dis, Key Lab Organ Regenerat & Transplantat,Minist Educ, Changchun, Jilin, Peoples R China

2.Jilin Agr Univ, Coll Life Sci, Lab Pathogen Microbiol & Immunol, Changchun, Jilin, Peoples R China

3.First Hosp Jilin Univ, Neurosci Ctr, Dept Neurol, Changchun, Jilin, Peoples R China

4.Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Jilin, Peoples R China

5.Hulunbuir Anim Dis Control Ctr, Hailar, Inner Mongolia, Peoples R China

关键词： bovine hepacivirus (BovHepV); sheep; cattle; phylogenetic evolution; China

期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：4.8； 五年影响因子：5.5 ）

ISSN： 2235-2988

年卷期： 2025 年 15 卷

页码：

收录情况： SCI

摘要： Background Bovine hepacivirus (BovHepV) is a recently identified member of the expanding genus Hepacivirus within the family Flaviviridae. However, the genetic diversity, geographical distribution, and host range of the virus remains poorly understood.Methods In this study, serum samples from cattle and sheep were obtained in Hulunbuir and pooled to establish RNA libraries, which were then analyzed using transcriptome sequencing. BovHepV-positive samples were confirmed using semi-nested PCR with primers designed based on the obtained viral sequences. Comprehensive bioinformatics analyses were employed to assess sequence similarity, phylogenetic evolution, and recombination of the obtained viral strains.Results A total of 988 serum samples from sheep (520) and cattle (468) were collected from 12 administrative districts in Hulunbuir from June to August, 2022. Semi-nested PCR revealed 6 BovHepV-positive districts with prevalence ranging from 2.0% to 35.0% in cattle, and one BovHepV-positive district with prevalence of 2.5% in sheep. The nucleotide sequence identities between viral strains from sheep and cattle ranged from 91.3% to 93.8%, while the amino acid sequence identities were between 95.4% and 96.7%. Phylogenetic analyses classified the obtained BovHepV strains within subtype G, genotype 1. Recombination analysis revealed the intergenerational relationships among the viral strains obtained from cattle and sheep.Conclusion We identified genetic diversity in subtype G strains in cattle and detected a BovHepV strain in a sheep for the first time in northeastern China, confirming cross-species transmission and co-circulation between cattle and sheep, thus expanding the virus's host range.

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