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A globular protein exhibits rare phase behavior and forms chemically regulated orthogonal condensates in cells

文献类型： 外文期刊

作者： Nie, Jinglei 1 ; Zhang, Xinyi 1 ; Hu, Zhijuan 1 ; Wang, Wei 3 ; Schroer, Martin A. 6 ; Ren, Jie 8 ; Svergun, Dmitri 7 ; Chen, Anyang 1 ; Yang, Peiguo 1 ; Zeng, An-Ping 1 ;

作者机构： 1.Westlake Univ, Ctr Synthet Biol & Integrated Bioengn, Hangzhou, Zhejiang, Peoples R China

2.Westlake Univ, Sch Life Sci, Hangzhou, Zhejiang, Peoples R China

3.Hamburg Univ Technol, Inst Bioproc & Biosyst Engn, Hamburg, Germany

4.Westlake Univ, Res Ctr Ind Future, Hangzhou, Zhejiang, Peoples R China

5.Westlake Univ, Sch Engn, Zhejiang Key Lab Intelligent Low Carbon Synthet Bi, Hangzhou, Zhejiang, Peoples R China

6.Univ Duisburg Essen, Nanoparticle Proc Technol NPPT, Duisburg, Germany

7.Hamburg Outstat DESY, European Mol Biol Lab EMBL, Hamburg, Germany

8.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests,Minist, Key Lab Control Biol Hazard Factors Plant Origin A, Beijing, Peoples R China

9.BIOSAXS GmbH, Hamburg, Germany

期刊名称：NATURE COMMUNICATIONS （ 影响因子：15.7； 五年影响因子：17.2 ）

ISSN：

年卷期： 2025 年 16 卷 1 期

页码：

收录情况： SCI

摘要： Proteins with chemically regulatable phase separation are of great interest in the fields of biomolecular condensates and synthetic biology. Intrinsically disordered proteins (IDPs) are the dominating building blocks of biomolecular condensates which often lack orthogonality and small-molecule regulation desired to create synthetic biomolecular condensates or membraneless organelles (MLOs). Here, we discover a well-folded globular protein, lipoate-protein ligase A (LplA) from E. coli involved in lipoylation of enzymes essential for one-carbon and energy metabolisms, that exhibits structural homomeric oligomerization and a rare LCST-type reversible phase separation in vitro. In both E. coli and human U2OS cells, LplA can form orthogonal condensates, which can be specifically dissolved by its natural substrate, the small molecule lipoic acid and its analogue lipoamide. The study of LplA phase behavior and its regulatability expands our understanding and toolkit of small-molecule regulatable protein phase behavior with impacts on biomedicine and synthetic biology.

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