Development of SNP marker panels for genotyping by target sequencing (GBTS) and its application in soybean
文献类型: 外文期刊
第一作者: Yang, Qing
作者: Yang, Qing;Shi, Xiaolei;Qin, Jun;Zhang, Mengchen;Yang, Chunyan;Yan, Long;Zhang, Jianan;Chen, Lei;Song, Qijian
作者机构:
关键词: Soybean breeding; Marker-assisted selection (MAS); SNP marker panel; Genotyping by target sequencing (GBTS); Germplasm evaluation; QTL identification
期刊名称:MOLECULAR BREEDING ( 影响因子:3.1; 五年影响因子:3.1 )
ISSN: 1380-3743
年卷期: 2023 年 43 卷 4 期
页码:
收录情况: SCI
摘要: A high-throughput genotyping platform with customized flexibility, high genotyping accuracy, and low cost is critical for marker-assisted selection and genetic mapping in soybean. Three assay panels were selected from the SoySNP50K, 40K, 20K, and 10K arrays, containing 41,541, 20,748, and 9670 SNP markers, respectively, for genotyping by target sequencing (GBTS). Fifteen representative accessions were used to assess the accuracy and consistency of the SNP alleles identified by the SNP panels and sequencing platform. The SNP alleles were 99.87% identical between technical replicates and 98.86% identical between the 40K SNP GBTS panel and 10x resequencing analysis. The GBTS method was also accurate in the sense that the genotypic dataset of the 15 representative accessions correctly revealed the pedigree of the accessions, and the biparental progeny datasets correctly constructed the linkage maps of the SNPs. The 10K panel was also used to genotype two parent-derived populations and analyze QTLs controlling 100-seed weight, resulting in the identification of the stable associated genetic locus Locus_OSW_06 on chromosome 06. The markers flanking the QTL explained 7.05% and 9.83% of the phenotypic variation, respectively. Compared with GBS and DNA chips, the 40K, 20K, and 10K panels reduced costs by 5.07% and 58.28%, 21.44% and 65.48%, and 35.74% and 71.76%, respectively. Low-cost genotyping panels could facilitate soybean germplasm assessment, genetic linkage map construction, QTL identification, and genomic selection.
分类号:
- 相关文献
作者其他论文 更多>>
-
Recent advances in glycoside hydrolase family 20 and 84 inhibitors: Structures, inhibitory mechanisms and biological activities
作者:Jiang, Xi;Yang, Qing;Yang, Qing
关键词:Glycoside hydrolase; Hex; OGA; Inhibitor; Inhibitory mechanism; Biological activity
-
Effects of walnut seed coat polyphenols on walnut protein hydrolysates: Structural alterations, hydrolysis efficiency, and acetylcholinesterase inhibitory capacity
作者:Su, Guowan;Chen, Jieqiong;Huang, Lin;Zhao, Mouming;Zhang, Jianan;Deng, Liuxin;Chen, Jieqiong;Zhang, Yehui;Zhao, Tiantian;Huang, Qingrong;Zhao, Tiantian;Zhao, Mouming;Zhao, Tiantian;Zeng, Xi
关键词:Walnut hydrolysates; Seed coat polyphenols; Structural alterations; Hydrolysis efficiency; AChE inhibition; Synergistic effects
-
Transcriptome mapping related genes encoding PR1 protein involved in necrotic symptoms to soybean mosaic virus infection
作者:Zhao, Tiantian;Wang, Fengmin;Chen, Qiang;Liu, Luping;Yan, Long;Yang, Yue;Meng, Qingmin;Huang, Jinan;Zhang, Mengchen;Lin, Jing;Qin, Jun;Zhang, Yuhang;Zhang, Bo
关键词:Necrotic response; Transcriptome; Pathogenesis-related protein 1 (PR1); Soybean mosaic virus (SMV); Bulk segregant analysis (BSA)
-
Production of the antifungal biopesticide physcion through the combination of microbial fermentation and chemical post-treatment
作者:Zhuang, Zheng;Zhong, Xueqing;Li, Qinghua;Lin, Guo-Qiang;He, Qing-Li;Zhao, Qunfei;Yang, Qing;Liu, Wen;Liu, Tian
关键词:Antifungal biopesticide; Physcion; Microbial production
-
Improving crop-livestock integration in China using numerical experiments at catchment and regional scales
作者:Chen, Lei;Wang, Yiwen;Yang, Nian;Zhu, Kaihang;Yan, Xiaoman;Shen, Zhenyao;Bai, Zhaohai;Zhai, Limei
关键词:Agriculture; Crop -livestock integration; Fertilizer; Crop yield; Nonpoint source pollution
-
Verticillium dahliae Effector VdCE11 Contributes to Virulence by Promoting Accumulation and Activity of the Aspartic Protease GhAP1 from Cotton
作者:Li, Chi;Qin, Jun;Huang, Yingqi;Shang, Wenjing;Hu, Xiaoping;Li, Chi;Qin, Jun;Huang, Yingqi;Shang, Wenjing;Hu, Xiaoping;Chen, Jieyin;Chen, Jieyin;Klosterman, Steven J.;Subbarao, Krishna V.
关键词:Verticillium dahliae; effector; plant immunity; aspartic protease
-
Strongly-adhesive easily-detachable carboxymethyl cellulose aerogel for noncompressible hemorrhage control
作者:Pan, Siwen;Li, Yige;Chen, Lei;Wang, Li;Zhang, Qiang;Tong, Xinru;Li, Tao
关键词:Hemostatic aerogel; Covalent adhesion; Easy to remove; Antibacterial; Noncompressible hemorrhage