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Osteogenic Activity of MG63 Cells on Hydroxyapatite/Collagen Nanocomposite Membrane

文献类型: 会议论文

第一作者: Masanori Kikuchi

作者: Masanori Kikuchi 1 ;

作者机构: 1.Biomaterials Center, National Institute for Materials Science, 1-1, Namiki, Tsukuba, Ibaraki 305-0044, Japan

关键词: hydroxyapatite;collagen;nanocomposite;membrane;osteogenic activity

会议名称: Bioceramics vol.19 pt.1

主办单位:

页码: 311,316-

摘要: The hydroxyapatite/collagen (HAp/Col) nanocomposite membrane was prepared from the HAp/Col nanocomposite fibers. The HAp/Col membrane crosslinked by a vacuum heating showed 14±2 (dry) and 1.0±0.1 (wet) MPa in tensile strengths that were enough strength to operate in both cell culture and surgical operations. Histological observation and total DNA amount analysis demonstrated that human osteoblastic cells, MG63, derived from osteosarcoma on the HAp/Col membrane adhered and proliferated well as the same as those on tissue culture polystyrene (TCPS). Real-time polymerase chain reaction analyses indicated that alkaline phosphatase gene expression on the HAp/Col membrane was 1.4 times greater than that on TCPS. These results suggested that the HAp/Col nanocomposite membrane can be utilized in bone tissue engineering scaffold as well as bone filler.

分类号: [ "R318.08" , "R318.08"]

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