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Quantitative Analysis of Core Binding Factor 1 (Cbfal) and Osteocalcin in C2C12 Cells Induced by Ca/P Ceramics in vitro

文献类型： 会议论文

第一作者： Yanfei Tan

作者： Yanfei Tan 1 ; Gang Wang 2 ; Hongsong Fan 3 ; Xinlong Wang; Jian Lu; Xingdong Zhang;

作者机构： 1.Engineering research center for biomaterials, Sichuan Univ. Chengdu 610064 China

2.National Engineering Research Center for Biomaterials, Chengdu 610064 China

3.College of Life science, Sichuan Univ. Chengdu 610064 China

关键词： osteoinduction;calcium phosphate ceramics;cbfal;osteocalcin;C2C12 cells

会议名称： Bioceramics vol.19 pt.2

主办单位：

页码： 1067,1070-

摘要： The mRNA expression of Cbfal and osteocalcin gene induced by calcium phosphate ceramics (Ca/P) were quantitative analyzed according to real-time RT-PCR method in this work. C2C12 cells were co-culture with four kinds of porous Ca/P ceramics for 2 and 5d without adding other growth factors. The four kinds of Ca/P ceramics were pure hydroxyapatite (HA) sintered at 1250℃ and HA/TCP with a ratio of 60/40 sintered at 1100℃ (HT1), 1200℃ (HT2) and 1250℃ (HT3) respectively. Real-time RT-PCR analysis found the Ca/P ceramics induced positive expression of Cbfal and osteocalcin in C2C12 cells, After 5 days culture, Cbfal and osteocalcin showed obvious higher expression compared with that in 2 days. Cbfal and osteocalcin expression in BCP was much higher than HA, and the expression level of osteocalcin was HT1 > HT2 > HT3 > HA. Our results showed that Ca/P ceramics alone were sufficient to induce C2C12 cells to osteoblastic differentiation and the sinter temperature and phase composition of Ca/P ceramics could affect their osteoindctive capacity significantly.

分类号： [ "R318.08" , "R318.08"]

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