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Effect of Acid Treated Hydroxyapatite on Osteoblast Maturation

文献类型: 会议论文

第一作者: Taro Takemura

作者: Taro Takemura 1 ; Toshiyuki Ikoma; Nobutaka Hanagata;

作者机构: 1.Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan

关键词: hydroxyapatite;osteoblast;mineralization;acid;gene expression

会议名称: International Symposium on Ceramics in Medicine;International Society for Ceramics Annual Meeting; 20071024-26;20071024-26; Nantes(FR);Nantes(FR)

主办单位:

页码: 1029,1032-

摘要: Surfaces of sintered hydroxyapatite (HAp) were treated with four kinds of acids, hydrochloric acid (HCl), nitric acid (HNO_3), sulfuric acid (H_2SO_4) and hydrogen peroxide (H_2O_2). On these acid-treated HAp surfaces, osteoblast-like MC3T3-E1 cells were cultured for 20 days. The matrix mineralization was observed in HCl-treated HAp and HNO_3-treated HAp, but not in non-treated HAp, H_2SO_4-treated HAp and H_2O_2-treated HAp. Gene expressions of Bglap2 encoding osteocalcin and Akp2 encoding alkaline phosphatase were higher in HCl-treated HAp and HNO_3-treated HAp than those in the others. These results indicate that not only hydrochloric acid but also nitric acid improves performance of HAp to mature osteoblast.

分类号: [ "R318.08" , "R318.08"]

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