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Studies on the difference of the merozoites proteins of babesia major and babesia bigemina

文献类型： 会议论文

第一作者： Yin Hong

作者： Yin Hong 1 ; Lu Wenshun; Luo Jianxun; Zhang Qicai; Lu Wenxiang; Dou Huifang;

作者机构： 1.Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 740046, P. R. China

会议名称： Research progress in animal industry and animal products processing

主办单位：

页码： 371-374

摘要： Three splenectomized calves were infected by inoculation of Babesia major (xinjiang strain), B. major (Henan strain) and B. bigemina stabilates. The merozoites were purified from bovine erythrocytes through CF-11 cellulose, AH-haemolysin, percoll and centrifugation. These merozoites were analyzed by SDS-PAGE and Western blot. The pattern of SDS-PAGE of the merozoites proteins rebealed that the proteins components of both strains of B. major were very similar, but difference from that of B. bigemina. The merozoites of B. major (Xinjiang) expressed 16 main bands, and R major(Henan) 17 bands, B. bigemina 10 bands. The Western blot shown that the proteins of all the three strains of Babesia could be recognized by the antisera produced from three cattle injected with the three Babesia strains respectively. Several major babesial antigen bands have been demonstrated. 28, 18 kDa proteins were the immunodominant proteins of both strains of B. major and the 18 kDa epitope was the strongest. Therefore the18 kDa maybe the potential protein for molecular vaccine.

分类号： S8

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