Genetically-encoded targeted protein degradation technology to remove endogenous condensation-prone proteins and improve crop performance
文献类型： 外文期刊
作者： Luo, Ming 1 ; Zhu, Sitao 1 ; Dang, Hua 1 ; Wen, Qing 1 ; Niu, Ruixia 1 ; Long, Jiawei 1 ; Wang, Zhao 1 ; Tong, Yongjia 1 ; Ning, Yuese 4 ; Yuan, Meng 2 ; Xu, Guoyong 1 ;
作者机构： 1.Wuhan Univ, Inst Adv Studies IAS, State Key Lab Hybrid Rice, Wuhan, Hubei, Peoples R China
2.Hubei Hongshan Lab, Wuhan, Hubei, Peoples R China
3.Wuhan Univ, RNA Inst, Wuhan, Hubei, Peoples R China
4.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing, Peoples R China
5.Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Natl Key Lab Crop Genet Improvement, Wuhan, Hubei, Peoples R China
期刊名称：NATURE COMMUNICATIONS （ 影响因子：15.7； 五年影响因子：17.2 ）
ISSN：
年卷期： 2025 年 16 卷 1 期
页码：
收录情况： SCI
摘要： Effective modulation of gene expression in plants is achievable through tools like CRISPR and RNA interference, yet methods for directly modifying endogenous proteins remain lacking. Here, we identify the E3 ubiquitin ligase E3TCD1 and develope a Targeted Condensation-prone-protein Degradation (TCD) strategy. The X-E3TCD1 fusion protein acts as a genetically engineered degrader, selectively targeting endogenous proteins prone to condensation. For example, a transgenic E3TCD1 fusion with Teosinte branched 1 (TB1) degrades the native TB1 protein, resulting in increased tiller numbers in rice. Additionally, conditional degradation of the negative defense regulator Early Flowering 3 via a pathogen-responsive ProTBF1-uORFsTBF1 cassette enhances rice blast resistance without affecting flowering time in the absence of pathogen. Unlike prevailing targeted protein degradation strategies, the TCD system does not rely on small molecules, antibodies, or genetic knock-in fusion tags, demonstrating its promise as a transgene-based approach for optimizing crop performance.
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