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Peptide aptamer-based polyaniline-modified amperometric biosensor for L-lysine detection in real serum samples

文献类型： 外文期刊

作者： Wang, Wenjing 1 ; He, Yumin 1 ; Deng, Lei 4 ; Wang, Hui 5 ; Liu, Xiaoying 2 ; Gui, Qing-wen 2 ; Cao, Zhong 6 ; Feng, Zemeng 1 ; Xiong, Benhai 5 ; Yin, Yulong 1 ;

作者机构： 1.Chinese Acad Sci, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry P, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Inst Subtrop Agr,Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China

2.Hunan Agr Univ, Coll Chem & Mat Sci, Changsha 410128, Hunan, Peoples R China

3.Univ South China, Hengyang Med Sch, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China

4.Cent South Univ, Sch Comp Sci & Engn, Changsha 410075, Hunan, Peoples R China

5.Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing 100193, Peoples R China

6.Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect Power & Trans, Changsha 410114, Hunan, Peoples R China

7.Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Cytochem, Changsha 410114, Hunan, Peoples R China

关键词： L-Lys; Polyaniline; Peptide aptamer; Biosensor

期刊名称：MEASUREMENT （ 影响因子：5.6； 五年影响因子：5.0 ）

ISSN： 0263-2241

年卷期： 2023 年 221 卷

页码：

收录情况： SCI

摘要： L-Lysine (L-Lys) quantification in serum using a novel and highly selective amperometric biosensor has been reported. In this study, an efficient, enzyme-free, and simple anti-fouling biosensor was developed based on a self-screened peptide aptamer. A glassy carbon electrode (GCE) was used to construct anti-fouling interfaces by modifying its surface with polyaniline (PANI) polymer. The peptide aptamer, Cys-Pro-Pro-Pro-Pro-Arg-Glu-AsnIle-Gln-Arg-Leu-Thr, was then immobilized onto this electrode via an electroactive cysteine linker and its potential in the determination of L-Lys was examined. Under optimised experimental conditions, the peptide modified electrodes exhibited excellent anti-fouling and electrochemical sensing. The biosensor was effective in resisting biofouling in a wide range of serum samples and amino acid solutions, and its linear range for L-Lys detection ranged from 1 nM to 10 mM, with a comparatively lower detection limit (0.3 nM; S/N = 3). The antifouling biosensor could detect L-Lys in real serum samples, and this approach of designing peptide aptamers based low-fouling biosensors can easily be extended to the development of a bio-sensing platform system for a variety of other metabolites.

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