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Electrochemiluminescence Resonance Energy Transfer Biosensor for the Human-Associated Clade of Streptococcus suis Based on Prereduction-Enhanced Yttrium MOFs

文献类型： 外文期刊

作者： Hu, Can 1 ; Xiang, Hongzhang 1 ; Yin, Yashi 1 ; Li, Jiawen 1 ; Zhang, Yutian 1 ; Huang, Xingao 1 ; Guo, Yating 1 ; He, Yingsi 1 ; Gao, Yanru 1 ; Ren, Kejing 1 ; Han, Heyou 1 ; Wang, Wenjing 1 ; Li, Jinquan 1 ;

作者机构： 1.Huazhong Agr Univ, Natl Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

2.Huazhong Agr Univ, Coll Life Sci & Technol, Hubei Hongshan Lab, Wuhan 430070, Peoples R China

3.Huazhong Agr Univ, Coll Chem, Wuhan 430070, Peoples R China

4.Chinese Acad Agr Sci, Shenzhen Branch, Guangdong Lab Lingnan Modern Agr, Genome Anal Lab,Minist Agr & Rural Affairs,Agr Gen, Shenzhen 518000, Peoples R China

5.Guangdong Med Univ, Affiliated Hosp, Lab Hepatobiliary Surg, Zhanjiang 524001, Peoples R China

期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：6.7； 五年影响因子：6.6 ）

ISSN： 0003-2700

年卷期： 2025 年 97 卷 5 期

页码：

收录情况： SCI

摘要： Streptococcus suis, a significant zoonotic pathogen, annually caused substantial economic losses in the swine industry and had intensified threat to public health due to the recent emergence of human-associated clade. In this study, we discovered that the rare-earth metal-based metal-organic frameworks (Y-BTC) possessed excellent ECL capabilities. After prereduction at high voltage, its ECL intensity was enhanced by two times. Subsequently, we developed an efficient CRISPR/Cas12a-mediated electrochemiluminescence resonance energy transfer (ECL-RET) biosensor utilizing Y-BTC for the detection of the human-associated S. suis clade. Y-BTC was employed as the ECL-RET donor and ECL emitter, and the spherical nucleic acid Au NP was utilized as the ECL-RET receptor. In the presence of the target, isothermal amplification was triggered to generate a large number of amplicons, which subsequently activated the trans-cleavage activity of Cas12a. Cas12a cleaved the nucleic acid shell on the surface of Au NPs, reducing the spatial distance between Au NPs and Y-BTC due to electrostatic adsorption, thereby quenching the ECL of Y-BTC via ECL-RET. Consequently, the presence of targets can be observed by a reduced ECL signal. The sensor exhibited a detection range of 25 pM to 50 nM, with a detection limit as low as 17 pM. The practical utility was verified through actual sample testing. Our proposed ECL-RET sensing strategy provides a new avenue for the sensitive detection of S. suis. The universality has also been demonstrated using Fusobacterium nucleatum, Salmonella pullorum, and Listeria monocytogenes, holding great promise in the field of food safety and public health.

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