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Dynamic interplay between RNA N6-methyladenosine modification and porcine reproductive and respiratory syndrome virus infection

文献类型： 外文期刊

作者： Wang, Zi-Han 1 ; Li, Jing 1 ; Ma, Sai-Ya 1 ; Liu, Meng-Xuan 1 ; Zhan, Yu-Fei 1 ; Jin, Feng 1 ; Liu, Bing-Xin 1 ; Wang, Wenjing 1 ; He, Mei 1 ; Yang, Yu-Chuan 2 ; Tang, Yandong 3 ; Wang, Peng 4 ; Zhang, Wuchao 2 ; Tong, Jie 1 ;

作者机构： 1.Hebei Univ, Coll Life Sci, Sch Life Sci & Green Dev, Baoding 071002, Peoples R China

2.Hebei Agr Univ, Coll Vet Med, Baoding 071001, Peoples R China

3.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Anim Dis Control & Prevent, Harbin 045100, Peoples R China

4.Hebei Prov Hosp Tradit Chinese Med, Shijiazhuang 050000, Peoples R China

5.Hebei Univ Chinese Med, Neural Acad Tradit Chinese Med, Shijiazhuang 050000, Peoples R China

关键词： N-6-methyladenosine (m(6)a); porcine reproductive and respiratory syndrome virus (PRRSV); virus replication; m(6)A RNA immunoprecipitation sequencing (m(6)A-seq); p38/MAPK signalling pathway

期刊名称：VETERINARY RESEARCH （ 影响因子：3.5； 五年影响因子：4.0 ）

ISSN： 0928-4249

年卷期： 2025 年 56 卷 1 期

页码：

收录情况： SCI

摘要： N-6-methyladenosine (m(6)A) has attracted significant attention for its role in regulating the complex interaction between viruses and host cells. Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant pathogen affecting swine health worldwide. Here, we first identified seven m(6)A-enriched peaks in PRRSV genomic RNA by m(6)A RNA immunoprecipitation sequencing (m(6)A-seq). Moreover, functional analyses revealed a positive correlation between the m(6)A modification level and PRRSV replication. Treatment with the universal methylation inhibitor 3-deazaadenosine (3-DAA) effectively suppressed PRRSV replication in a dose-dependent manner. Furthermore, m(6)A-seq was also used to determine the m(6)A landscape of the transcriptome in PAMs infected with pandemic or highly pathogenic PRRSV strains. Among the 4677 transcripts exhibiting altered m(6)A modification levels, the MAPK14 gene and the p38/MAPK signalling pathway emerged as preliminary targets of m(6)A-mediated epigenetic regulation during PRRSV infection. These findings provide new insights into the epigenetic mechanisms underlying PRRSV infection and may facilitate the development of anti-PRRSV therapeutics.

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