Monogalactosyldiacylglycerol synthase isoforms play diverse roles inside and outside the diatom plastid
文献类型： 外文期刊
作者： Gueguen, Nolwenn 1 ; Seres, Yannick 1 ; Ciceron, Felix 1 ; Gros, Valerie 1 ; Larbi, Gregory Si 1 ; Falconet, Denis 1 ; Deragon, Etienne 1 ; Gueye, Siraba D. 1 ; Le Moigne, Damien 1 ; Schilling, Marion 1 ; Cussac, Mathilde 1 ; Petroutsos, Dimitris 1 ; Hu, Hanhua 3 ; Gong, Yangmin 4 ; Michaud, Morgane 1 ; Jouhet, Juliette 1 ; Salvaing, Juliette 1 ; Amato, Alberto 1 ; Marechal, Eric 1 ;
作者机构： 1.Univ Grenoble Alpes, CNRS, Commissariat Energie Atom & Energies Alternat, Lab Physiol Cellulaire & Vegetale,Inst Natl Rech A, 17 rue Martyrs, F-38000 Grenoble, France
2.Uppsala Univ, Dept Organismal Biol, S-75236 Uppsala, Sweden
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
4.Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Peoples R China
5.Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr, Wuhan 430062, Peoples R China
6.Exeter Univ, Living Syst Inst, Stocker Rd, Exeter EX4 4QD, England
7.Inst Biomol Max Mousseron, 1919 Route Mende, F-34293 Montpellier, France
期刊名称：PLANT CELL （ 影响因子：11.6； 五年影响因子：12.1 ）
ISSN： 1040-4651
年卷期： 2024 年
页码：
收录情况： SCI
摘要： Diatoms derive from a secondary endosymbiosis event, which occurred when a eukaryotic host cell engulfed a red alga. This led to the formation of a complex plastid enclosed by 4 membranes: 2 innermost membranes originating from the red alga chloroplast envelope, and 2 additional peri- and epiplastidial membranes (PPM and EpM). The EpM is linked to the endoplasmic reticulum (ER). The most abundant membrane lipid in diatoms is monogalactosyldiacylglycerol (MGDG), synthesized by galactosyltransferases called MGDG synthases (MGDs), conserved in photosynthetic eukaryotes and considered to be specific to chloroplast membranes. Similar to angiosperms, a multigenic family of MGDs has evolved in diatoms, but through an independent process. We characterized MGD alpha, MGD beta, and MGD gamma in Phaeodactylum tricornutum, combining molecular analyses, heterologous expression in Saccharomyces cerevisiae, and studying overexpressing and CRISPR-Cas9-edited lines. MGD alpha localizes mainly to thylakoids, MGD beta to the PPM, and MGD gamma to the ER and EpM. MGDs have distinct specificities for diacylglycerol, consistent with their localization. The results suggest that MGD alpha is required for thylakoid expansion under optimal conditions, while MGD beta and MGD gamma play roles in plastid and nonplastid membranes and in response to environmental stress. Functional compensation among MGDs likely contributes to diatom resilience under adverse conditions and to their ecological success. A multigenic family of membrane-localized monogalactosyldiacylglycerol synthases is present in diatoms, with specific isoforms for thylakoid expansion and galactolipid production in response to stress.
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