Functional study of Phaeodactylum tricornutum Seipin highlights specificities of lipid droplets biogenesis in diatoms
文献类型： 外文期刊
作者： Le Moigne, Damien 1 ; Carletti, Marta 1 ; Amari, Chems 1 ; Bonnarde, Rachel 1 ; Richard, Fabien 1 ; Albrieux, Catherine 1 ; Mahieu, Megane 1 ; Arrighi, Matteo 1 ; Si-Larbi, Gregory 1 ; Jouneau, Pierre-Henri 4 ; Gros, Valerie 1 ; Louwagie, Mathilde 1 ; Roy, Sylvaine 1 ; Gong, Yangmin 5 ; Pan, Yufang 6 ; Hu, Hanhua 6 ; Bastien, Olivier 1 ; Jouhet, Juliette 1 ; Amato, Alberto 1 ; Marechal, Eric 1 ; Salvaing, Juliette 1 ;
作者机构： 1.Univ Grenoble Alpes, Inst Natl Rech Agr Alimentat & Environm, Ctr Natl Rech Sci, Lab Physiol Cellulaire & Vegetale,Commissariat Ene, 17 Rue Martyrs, F-38000 Grenoble, France
2.CEA Grenoble, IRIG, 17 Rue Martyrs, F-38000 Grenoble, France
3.Sorbonne Univ, PSL Univ, CNRS, CPCV,Dept Chem,Ecole Normale Super, F-75005 Paris, France
4.Univ Grenoble Alpes, Lab Modelisat & Explorat Mat, Commissariat Energie Atom & Energies Alternat, IRIG,CEA Grenoble, 17 Ave Martyrs, F-38000 Grenoble, France
5.Chinese Acad Agr Sci, Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, Oil Crops Res Inst, Wuhan 430062, Peoples R China
6.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
关键词：
lipid droplets biogenesis; lipidomics;
期刊名称：NEW PHYTOLOGIST （ 影响因子：8.1； 五年影响因子：10.3 ）
ISSN： 0028-646X
年卷期： 2025 年 247 卷 5 期
页码：
收录情况： SCI
摘要： Diatoms are a major phylum of microalgae, playing crucial ecological roles. They derive from secondary endosymbiosis, leading to a complex intracellular architecture. Their ability to store oil in lipid droplets (LDs) upon unfavourable conditions has raised interest for applications, in particular biofuels, yet Lipid Droplet (LD) biogenesis mechanisms in these organisms remain poorly understood. Here, we functionally characterize the homolog of Seipin, a major actor of LD biogenesis, in Phaeodactylum tricornutum. We used an in silico approach to analyze the evolutionary origin of PtSeipin and its specific features. Then, we used a functional genetics approach with a combination of confocal and electronic microscopy and lipidomics to characterize the protein function. We provide evidence that Stramenopiles Seipins were inherited from the host during secondary endosymbiosis. The localization of PtSeipin highlights participation of the plastid's most external membrane in LD biogenesis. Finally, the knock-out (KO) of PtSeipin leads to a strong increase of triacylglycerol (TAG) accumulation, a feature that had not been observed in adipogenic or oleaginous cells and is greatly enhanced following high light exposure. Our results suggest a redirection of lipid fluxes toward TAG synthesis, reduced TAG recycling or a combination of both in PtSeipin KO.
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