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Delta-5 elongase knockout reduces docosahexaenoic acid and lipid synthesis and increases heat sensitivity in a diatom

文献类型： 外文期刊

作者： Zhu, Junkai 1 ; Li, Shuangqing 1 ; Chen, Weizhong 1 ; Xu, Xinde 4 ; Wang, Xiaoping 1 ; Wang, Xinwei 1 ; Han, Jichang 6 ; Jouhet, Juliette 7 ; Amato, Alberto 7 ; Marechal, Eric 7 ; Hu, Hanhua 8 ; Allen, Andrew E. 9 ; Gong, Yangmin 2 ; Jiang, Haibo 1 ;

作者机构： 1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Peoples R China

2.Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Peoples R China

3.Southern Marine Sci & Engn Guangdong Lab Zhuhai, Life & Ecol Ctr, Zhuhai 519080, Peoples R China

4.Zhejiang Med Co Ltd, Dept Human Nutr, Xinchang 312500, Peoples R China

5.Zhejiang Keming Biopharmaceut Co Ltd, Dept Human Nutr, Xinchang 312500, Peoples R China

6.Ningbo Univ, Coll Food Sci & Engn, Ningbo 315211, Peoples R China

7.Univ Grenoble Alpes, Ctr Natl Rech Sci Commissariat Energie Atom & Ener, Unite mixte Rech 5168, Lab Physiol Cellulaire & Vegetale,RIG,CEA Grenobl, F-38041 Grenoble, France

8.Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China

9.Univ Calif San Diego, Scripps Inst Oceanog, La Jolla, CA 92093 USA

10.J Craig Venter Inst, Dept Environm & Sustainabil, La Jolla, CA 92037 USA

期刊名称：PLANT PHYSIOLOGY （ 影响因子：6.5； 五年影响因子：7.6 ）

ISSN： 0032-0889

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Recent global marine lipidomic analysis reveals a strong relationship between ocean temperature and phytoplanktonic abundance of omega-3 long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which are essential for human nutrition and primarily sourced from phytoplankton in marine food webs. In phytoplanktonic organisms, EPA may play a major role in regulating the phase transition temperature of membranes, while the function of DHA remains unexplored. In the oleaginous diatom Phaeodactylum tricornutum, DHA is distributed mainly on extraplastidial phospholipids, which is very different from the EPA enriched in thylakoid lipids. Here, clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9-mediated knockout of delta-5 elongase (ptELO5a), which encodes a delta-5 elongase (ELO5) catalyzing the elongation of EPA to synthesize DHA, led to a substantial interruption of DHA synthesis in P. tricornutum. The ptELO5a mutants showed some alterations in transcriptome and glycerolipidomes, including membrane lipids and triacylglycerols under normal temperature (22 degrees C), and were more sensitive to elevated temperature (28 degrees C) than wild type. We conclude that PtELO5a-mediated synthesis of small amounts of DHA has indispensable functions in regulating membrane lipids, indirectly contributing to storage lipid accumulation, and maintaining thermomorphogenesis in P. tricornutum. This study also highlights the significance of DHA synthesis and lipid composition for environmental adaptation of P. tricornutum. Knockout of delta-5 elongase in Phaeodactylum tricornutum reveals docosahexaenoic acid composition in phospholipids indirectly promotes storage lipid accumulation and reduces heat sensitivity.

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