A novel HCR-CRISPR/Cas12a immunosensor for the sensitive detection of pesticide residues in animal-derived foods
文献类型： 外文期刊
作者： Wang, Yuanshang 1 ; Du, Pengfei 1 ; Wang, Xiaohong 2 ; Wang, Jia 2 ; Lu, Youyou 2 ; He, Feng 4 ; Wang, Haijie 5 ; Wang, Weiting 1 ; Liu, Yaobo 1 ; Wang, Shoujing 1 ; Cao, Jianfang 1 ; Ma, Yanli 1 ; Abd El-Aty, A. M. 7 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Food & Nutr Sci & Technol, Shandong Prov Key Lab Agroprod Proc Technol, Key Lab Novel Food Resources Proc,Minist Agr, Jinan 250100, Peoples R China
2.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China
3.Huazhong Agr Univ, Key Lab Environm Correlat Dietol, Wuhan 430070, Peoples R China
4.Jiangnan Univ, Sch Food Sci & Technol, Int Joint Res Lab Biointerface & Biodetect, Wuxi 214122, Peoples R China
5.Chinese Acad Agr Sci, Inst Food Sci & Technol, Key Lab Agroprod Proc, Minist Agr & Rural Affairs, Beijing 100193, Peoples R China
6.Univ Coll Dublin, Sch Agr & Food Sci, Dublin, Ireland
7.Cairo Univ, Fac Vet Med, Dept Pharmacol, Giza 12211, Egypt
8.Ataturk Univ, Med Fac, Dept Med Pharmacol, TR-25240 Erzurum, Turkiye
关键词： Hybridization chain reaction; CRISPR/Cas12a; Immunosensor; Pesticide residue; Animal-derived food; Gold nanoparticles
期刊名称：FOOD BIOSCIENCE （ 影响因子：5.9； 五年影响因子：6.1 ）
ISSN： 2212-4292
年卷期： 2024 年 62 卷
页码：
收录情况： SCI
摘要： Pesticides are widely used in the cultivation and breeding of agricultural products. However, pesticide residue monitoring has focused mainly on fruits and vegetables and has not focused on animal-derived foods. Therefore, a novel approach for determining pesticide residues in animal-derived food products via an immunosensor based on a chain hybridization reaction (HCR) with CRISPR/Cas12a is presented. Triazophos pesticide was used as a research object. This method establishes a competitive immunoreaction system in which OVA-hapten is encapsulated on the surface of the enzyme label plate, competing with pesticide molecules bound to antibodies on the surface of the nanogold composite probe. Integrating the immunoreaction with two signal amplification mechanisms, HCR and CRISPR/Cas12a, ensures heightened method sensitivity while maintaining enhanced convenience and speed. The method's detection range spans from 0.01 to 50 ng/mL, with a remarkably low detection limit of 0.013 ng/mL. Moreover, in practical sample applications, the assay exhibited a robust correlation with GC-MS. This approach uniquely offers real-time, cost-effective, and onsite data, providing a more comprehensive and timely foundation for establishing standards on pesticide residues in animal-derived foods, bolstering food safety surveillance, and upholding public health. Moreover, the proposed HCR-CRISPR/Cas12a immunosensor effectively improved the sensitivity and accuracy of pesticides detection, offering a promising and versatile platform for food safety detection.
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